| 摘要: |
| 目的 探讨微RNA-484（miR-484）对肝纤维化发生和发展过程中关键细胞肝星状细胞（HSC）的作用和影响。方法 以前期芯片筛选结果为基础，通过细胞转染人为干预miR-484在HSC-T6细胞株中的表达。利用qPCR、蛋白质印迹法检测肝纤维化及凋亡相关指标的表达变化；以Annexin Ⅴ-FITC/PI双染细胞后，用流式细胞仪检测miR-484对细胞凋亡的影响。结果 与对照组相比，人为下调miR-484在HSC-T6细胞株中的表达后，其靶基因Fis1及促凋亡因子caspase-3在mRNA和蛋白水平的表达均升高（P<0.05），凋亡抑制因子Bcl-2表达下降（P<0.05）；流式细胞仪检测到HSC-T6细胞凋亡率升高[（32.81±3.21）% vs（57.54±6.76）%，P<0.05]；同时肝纤维化相关指标α-平滑肌肌动蛋白、Ⅰ型胶原的mRNA和蛋白水平均下调（P<0.05）。结论 miR-484通过靶向Fis1抑制HSC凋亡、促进其活化，从而促进肝纤维化的发生和发展。 |
| 关键词: 微RNA-484 线粒体分裂蛋白1 肝纤维化 肝星状细胞 活化 细胞凋亡 |
| DOI：10.16781/j.0258-879x.2017.09.1146 |
| 投稿时间：2017-05-19修订日期：2017-06-24 |
| 基金项目:国家自然科学基金（81370553）. |
|
| MicroRNA-484 regulates liver fibrosis course through targeting Fis1 in hepatic stellate cells |
| ZHANG Li-fen△,LI Bin-bin△,YU Hong-yu* |
(Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
△Co-first authors
*Corresponding author) |
| Abstract: |
| Objective To explore the effects of microRNA-484 (miR-484) on hepatic stellate cells (HSCs), the key cell in the occurrence and development of liver fibrosis, and to investigate the functions. Methods On the basis of our previous microarray analysis results, we transfected HSC-T6 cell lines with miR-484 inhibitor to intervene the expression of miR-484 in vitro. The expressions of mRNA and proteins related to liver fibrosis and apoptosis were detected by qPCR and Western blotting, respectively. The cell apoptosis with Annexin Ⅴ-FITC/PI double staining was determined by flow cytometry. Results Compared with the control group, the mRNA and protein expression of miR-484-targeted gene Fis1 and proapoptotic factor caspase-3 were both significantly up-regulated (P<0.05), and apoptosis inhibitory factor Bcl-2 was significantly down-regulated (P<0.05) in the HSC-T6 cells transfected with miR-484 inhibitor; the apoptosis rate of HSC-T6 cells was significantly increased ([32.81±3.21]% vs[57.54±6.76]%, P<0.05), and α-smooth muscle actin and collagen type Ⅰ were both significantly down-regulated (P<0.05) in the HSC-T6 cells transfected with miR-484 inhibitor. Conclusion MiR-484 promotes the occurrence and progress of liver fibrosis through inhibiting the apoptosis and promoting the activation of HSCs by targeting Fis1. |
| Key words: microRNA-484 mitochondrial fission 1 protein liver fibrosis hepatic stellate cell activation apoptosis |
