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| 胃癌患者血浆24种氨基酸代谢物水平变化 |
| 修风民1,2,张凤3,矫健鹏1,张映城1,裴蓓1,赵婧1,修丽娟1*,岳小强1* |
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(1. 第二军医大学长征医院中医科, 上海 200003;
2. 东莞市塘厦医院普外一科, 东莞 523710;
3. 第二军医大学长征医院药材科, 上海 200003
*通信作者) |
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| 摘要: |
| 目的 研究胃癌患者血浆氨基酸代谢物中是否有可作为胃癌诊断的生物标志物。方法 采用超高液相色谱串联质谱（UHPLC-MS/MS）法检测血浆24种氨基酸水平，比较胃癌患者与正常人群、胃癌患者不同分期、胃癌患者术前与术后的变化。结果 胃癌患者血浆中丙氨酸（Ala）、甘氨酸（Gly）、谷氨酸（Glu）等18种氨基酸水平均较正常对照组下降，缬氨酸（Val）、精氨酸（Arg）、丝氨酸（Ser）水平较正常对照组升高，差异均有统计学意义（P均<0.05），对称性二甲基精氨酸（SDMA）、犬尿氨酸（Kyn）、马尿酸（HA）水平与正常对照组比较差异均无统计学意义（P均>0.05）。Ⅲ~Ⅳ期胃癌患者血浆中的Arg水平高于Ⅰ~Ⅱ期胃癌患者，谷氨酰胺（Gln）、Glu、甲硫氨酸（Met）、苯丙氨酸（Phe）水平低于Ⅰ~Ⅱ期胃癌患者，差异有统计学意义（P均<0.05）。18例胃癌患者术后血浆中亮氨酸（Leu）、Arg、瓜氨酸（Cit）水平分别较术前下降，Gln、赖氨酸（Lys）、Glu、Phe水平较术前升高，差异有统计学意义（P均<0.05）。结论 胃癌患者血浆氨基酸代谢物如Gln、Arg在胃癌早期预测中有重要意义。 |
| 关键词: 胃癌 氨基酸 代谢物 血浆 谷氨酰胺 精氨酸 |
| DOI：10.16781/j.0258-879x.2018.01.0062 |
| 投稿时间：2017-08-05修订日期：2017-09-06 |
| 基金项目:国家自然科学基金（81603434），上海市卫生和计划生育委员会项目（ZY3-CCCX-3-3056）. |
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| Variation of 24 plasma amino acid metabolite levels in patients with gastric cancer |
| XIU Feng-min1,2,ZHANG Feng3,JIAO Jian-peng1,ZHANG Ying-cheng1,PEI Bei1,ZHAO Jing1,XIU Li-juan1*,YUE Xiao-qiang1* |
(1. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Surgery(Ⅰ), Tangxia Hospital of Dongguan, Dongguan 523710, Guangdong, China;
3. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
*Corresponding author) |
| Abstract: |
| Objective To study whether plasma amino acid metabolites in patients with gastric cancer can be used as biomarkers for the diagnosis of gastric cancer. Methods The levels of 24 kinds of plasma amino acids were detected by ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), and were compared between patients with gastric cancer and normal controls, between patients with different stages of gastric cancer, and between gastric cancer patients before and after operation. Results The levels of 18 kinds of plasma amino acids including alanine (Ala), glycine (Gly) and glutamic acid (Glu) in gastric cancer patients were significantly lower than those in the normal controls, while the levels of valine (Val), arginine (Arg) and serine (Ser) were significantly higher than those in the normal controls (all P<0.05); there were no significant differences in symmetric dimethylarginine (SDMA), kynurenine (Kyn) or hippuric acid (HA) levels between the two groups (P>0.05). The plasma level of Arg in patients with Ⅲ-Ⅳ stage of gastric cancer was significantly higher than that in the Ⅰ-Ⅱ stage, while the plasma glutamine (Gln), Glu, methionine (Met) and phenylalanine (Phe) levels were significantly lower than those in the Ⅰ-Ⅱ stage (all P<0.05). The levels of plasma leucine (Leu), Arg and citrulline (Cit) in patients after operation were significantly lower than those before operation, while the plasma Gln, lysine (Lys), Glu and Phe levels were significantly higher than those before operation (all P<0.05). Conclusion Amino acids metabolites in plasma of patients with gastric cancer such as Gln and Arg play important roles in the early prediction of gastric cancer. |
| Key words: gastric carcinoma amino acid metabolite blood plasma glutamine arginine |
