| 摘要: |
| 目的 观察脊髓背角基质细胞衍生因子1（SDF-1）对皮肤肌肉切口牵拉术（SMIR）致持续性疼痛大鼠中枢敏感化及痛觉过敏的影响，为探讨术后慢性疼痛的发生机制和治疗靶点提供依据。方法 采用SMIR后持续性疼痛大鼠模型。将36只雄性SD大鼠随机分为假手术组（仅切开皮肤）和SMIR后1、5、10、20 d组以及SMIR+鞘内注射SDF-1中和性抗体组，每组6只大鼠。用up-down法测定术后大鼠痛行为学，用蛋白质印迹法检测各组大鼠脊髓组织中SDF-1的表达。再取18只雄性SD大鼠随机分为假手术组、SMIR组和SMIR+脊髓表面给予SDF-1中和性抗体组（每组6只大鼠），检测3组大鼠脊髓背角C纤维诱发场电位长时程增强（LTP）的变化。结果 与假手术组大鼠相比，SMIR后第5、10、20天大鼠脊髓组织SDF-1表达均增加（P均<0.05），且SMIR后大鼠痛阈降低（P<0.01），鞘内注射SDF-1中和性抗体可部分逆转SMIR引起的痛阈下降（P<0.05）。SMIR后1 h脊髓背角LTP升高（P<0.01），脊髓表面给予SDF-1中和性抗体可抑制SMIR导致的LTP升高（P<0.01）。结论 脊髓背角SDF-1参与了SMIR致持续性疼痛大鼠中枢敏感化及痛觉过敏，但具体机制有待进一步研究。 |
| 关键词: 手术后疼痛 基质细胞衍生因子1 中和性抗体 痛觉过敏 |
| DOI：10.16781/j.0258-879x.2018.05.0488 |
| 投稿时间：2017-11-29修订日期：2018-03-28 |
| 基金项目: |
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| Effects of stromal cell-derived factor 1 of spinal dorsal horn on central sensitivity and allodynia in rats with persistent pain evoked by skin/muscle incision and retraction |
| LI Xiu-juan△,LI Dai△,CHEN Hui,XIONG Yuan-chang* |
(Pain Center, Department of Anesthesiology, Changhai Hospital, Navy Medical University (Second Military Medical University), Shanghai 200433, China
△Co-first authors.
*Corresponding author) |
| Abstract: |
| Objective To explore the effects of stromal cell-derived factor 1 (SDF-1) on central sensitivity and allodynia in rats with skin/muscle incision and retraction (SMIR)-induced persistent pain, so as to provide reference for elucidating the potential mechanisms and therapeutic targets of postoperative chronic pain. Methods A postoperative chronic pain rat model was induced by SMIR. Thirty-six male SD rats were randomized into sham group, 1, 5, 10, and 20 d after SMIR groups and SMIR+intrathecal injection of SDF-1 neutralizing antibody group, with six rats in each group. The mechanical allodynia was determined with up-down method, and the expressions of SDF-1 were detected by Western blotting after surgery. Furthermore, 18 male SD rats were randomly divided into sham group (n=6), SMIR group (n=6) and SMIR+anti-SDF-1 group (SDF-1 neutralizing antibody was given on the surface of the spinal cord, n=6). The long-term potentiation (LTP) of C-fiber-evoked potentials in the rat spinal dorsal horn was detected in the three groups. Results The SDF-1 expressions were significantly increased on the 5, 10 and 20 days after SMIR versus the sham group (all P<0.05). The pain threshold of rats was significantly reduced after SMIR versus the sham group (P<0.01). Compared with the sham group, intrathecal injection of SDF-1 neutralizing antibody significantly suppressed the mechanical allodynia induced by SMIR (P<0.05). The LTP was significantly increased one hour after SMIR (P<0.01), and SDF-1 neutralizing antibody given on the surface of the spinal cord significantly inhibited the increased LTP induced by SMIR (P<0.01). Conclusion SDF-1 of the spinal dorsal horn is involved in SMIR-induced central sensitivity and allodynia in rats, but the specific mechanism is still unclear. |
| Key words: postoperatice pain stromal cell-derived factor 1 neutralizing antibody hyperalgesia |
