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髓过氧化物酶-抗中性粒细胞胞质抗体相关性血管炎患者血清IgG4及Th1/Th2细胞因子检测分析
秦陈浩,顾爱萍,展富琴,冯萍,顾昊宇,杜鸿*
0
(苏州大学附属第二医院检验科, 苏州 215004
*通信作者)
摘要:
目的 了解髓过氧化物酶(MPO)-抗中性粒细胞胞质抗体相关性血管炎(AAV)患者血清中IgG4及辅助性T细胞(Th) 1/Th2细胞因子的表达情况。方法 收集苏州大学附属第二医院50例AAV患者及40例同期健康体检者的血清样本,采用免疫比浊法测定IgG水平,采用ELISA法测定IgG4水平,采用流式细胞术微球阵列(CBA)技术检测血清中Th1/Th2细胞因子的水平,比较两组样本上述指标的差异,分析不同指标之间的相关性。结果 共收集到50例AAV患者血清样本,其中MPO-AAV患者40例(80.0%)。MPO-AAV组血清IgG4水平为(1.13±0.33) g/L,IgG4/IgG比值为0.094±0.032,IL-4水平为(2.54±0.68) pg/mL,IL-6水平为15.22(8.83,59.52) pg/mL,均高于健康体检者[IgG4水平为(0.80±0.27) g/L,IgG4/IgG比值为0.070±0.028,IL-4水平为(1.96±0.60) pg/mL,IL-6水平为4.95(2.82,13.25) pg/mL],差异均有统计学意义(P均<0.01),且MPO-AAV患者血清IgG4水平与IL-6水平呈正相关(rs=0.508,P<0.05)。结论 我院AAV患者中MPO-AAV比例较高。MPO-AAV患者血清中IgG4水平及IL-4、IL-6水平均升高,且IgG4升高与IL-6升高呈正相关,提示IgG4的升高与MPO-AAV患者Th1/Th2细胞因子平衡失调有关。
关键词:  免疫球蛋白G4  免疫球蛋白G  Th1/Th2细胞因子  抗中性粒细胞胞质抗体相关性血管炎  髓过氧化物酶
DOI:10.16781/j.CN31-2187/R.20190458
投稿时间:2019-05-18修订日期:2021-03-31
基金项目:
Detection and analysis of serum IgG4 and Th1/Th2 cytokines in patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis
QIN Chen-hao,GU Ai-ping,ZHAN Fu-qin,FENG Ping,GU Hao-yu,DU Hong*
(Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China
*Corresponding author)
Abstract:
Objective To investigate the expression of immunoglobulin (Ig) G4 and helper T cell (Th) 1/Th2 cytokines in the serum of patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis (MPOAAV).Methods Serum samples of 50 AAV patients and 40 healthy controls who taking a physical examination in the same period were collected from The Second Affiliated Hospital of Soochow University. The levels of IgG and IgG4 were detected by immunoturbidimetry and enzyme-linked immunosorbent assay, respectively. The expression of Th1/Th2 cytokines in serum was detected by flow cytometry microsphere array (CBA). The differences of the above indexes between the 2 groups were observed, and the correlations between different indexes were analyzed.Results Serum samples were collected from all the 50 AAV patients, including 40 of MPO-AAV patients (80.0%). In the MPO-AAV group, the serum IgG4 level was (1.13±0.33) g/L, IgG4/IgG ratio was 0.094±0.032, IL-4 level was (2.54±0.68) pg/mL, and IL-6 level was 15.22 (8.83, 59.52) pg/mL, which were significantly higher than those in the control group (IgG4: [ 0.80±0.27] g/L, IgG4/IgG ratio: 0.070±0.028, IL-4: [1.96±0.60] pg/mL and IL-6: 4.95 [2.82, 13.25] pg/mL; all P<0.01), and there was a positive correlation between the serum IgG4 and IL-6 levels in the MPO-AAV patients (rs=0.508, P<0.05).Conclusion The proportion of MPO-AAV patients is high in AAV patients in our hospital. The serum IgG4, IL-4 and IL-6 levels in MPO-AAV patients are significantly increased, and the level of IgG4 is positively correlated with the level of IL-6, suggesting that the increase of IgG4 is related to the imbalance of Th1/Th2 cytokines in MPO-AAV patients.
Key words:  immunoglobulin G4  immunoglobulin G  Th1/Th2 cytokines  anti-neutrophil cytoplasmic antibody-associated vasculitis  myeloperoxidase