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| 高压氧联合碱性成纤维细胞生长因子对煅烧牛骨修复大鼠骨缺损的作用及其与骨保护素、CD34表达的关系 |
| 陈铁楼1*,张新海1*,王世锋2,许兵3,陈骏1,马丽婷1,方以群2,刘进1 |
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(1. 海军军医大学(第二军医大学)长海医院全军口腔中心牙周科, 上海 200081;
2. 海军军医大学(第二军医大学)海军特色医学中心潜水与高气压医学研究室, 上海 200433;
3. 同济大学化学科学与工程学院普通化学教研室, 上海 200092
*通信作者) |
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| 摘要: |
| 目的 研究高压氧（HBO）联合碱性成纤维细胞生长因子（bFGF）对天然煅烧牛骨（CBB）修复骨缺损的作用，并初步探讨该作用与骨保护素（OPG）、CD34表达变化的关系。方法 32只SD大鼠随机分为两组，一组接受HBO治疗（HBO组，n=16），另一组不接受HBO治疗（NHBO组，n=16）。在大鼠左右侧顶骨处制作骨缺损，一侧移植CBB（CBB组）或CBB联合bFGF（CBBbF组），另一侧不移植作为对照（Con组）。HBO组接受2周HBO暴露，在术后4、8、12周时分别处死大鼠，通过影像学及组织学观察大鼠的骨缺损愈合情况，通过免疫组织化学染色观察骨缺损修复组织中OPG和CD34的表达情况。结果 术后12周时，HBO-CBB组和HBO-CBBbF组、NHBO-CBB组和NHBO-CBBbF组大鼠骨缺损区骨密度（BD）分别大于HBO-Con组和NHBO-Con组，HBO-CBBbF组BD大于其他各组（P均<0.01）。术后12周时，HBO-CBB组和HBO-CBBbF组、NHBO-CBB组和NHBO-CBBbF组新骨形成面积百分比分别大于HBO-Con组和NHBO-Con组（P均<0.01），血管数量分别多于HBO-Con组和NHBO-Con组。术后各时间点HBO-CBBbF组、HBO-CBB组和NHBO-CBBbF组血管数量均多于NHBO-CBB组。术后各时间点HBO-CBBbF组、HBO-CBB组和NHBO-CBBbF组OPG和CD34表达水平较NHBO-CBB组、HBO-Con组和NHBO-Con组升高（P<0.05，P<0.01）。结论 CBB对骨缺损修复有促进作用，bFGF可增加骨缺损修复中骨再生和血管数量，HBO对bFGF联合CBB修复骨缺损有协同作用，其机制与促进OPG和CD34表达有关。 |
| 关键词: 高压氧 煅烧牛骨 碱性成纤维细胞生长因子 骨再生 CD34抗原 骨保护素 |
| DOI：10.16781/j.0258-879x.2021.05.0519 |
| 投稿时间：2020-06-27修订日期：2020-09-27 |
| 基金项目:解放军总后勤部面上项目（CHJ13J035），上海市卫生和计划生育委员会科研课题（20134418）. |
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| Effect of hyperbaric oxygen combined with basic fibroblast growth factor on bone defect repair with calcined bovine bone in rats and its relationship with the expression of osteoprotegerin and CD34 |
| CHEN Tie-lou1*,ZHANG Xin-hai1*,WANG Shi-feng2,XU Bing3,CHEN Jun1,MA Li-ting1,FANG Yi-qun2,LIU Jin1 |
(1. Department of Periodontal, Oral Center of PLA, Changhai Hospital, Naval Medical University(Second Military Medical University), Shanghai 200081, China;
2. Department of Diving and Hyperbaric Medical Research, Naval Special Medical Center, Naval Medical University(Second Military Medical University), Shanghai 200433, China;
3. Department of General Chemistry, School of Chemical Science and Engineering, Tongji University, Shanghai 200092, China
*Corresponding authors) |
| Abstract: |
| Objective To investigate the effect of hyperbaric oxygen (HBO) combined with basic fibroblast growth factor (bFGF) on bone defect repair with natural calcined bovine bone (CBB), and to explore its relationship with the expression of osteoprotegerin (OPG) and CD34. Methods A total of 32 SD rats were randomly divided into two groups:HBO and non-HBO (NHBO) groups (n=16 in each group). Bone defects were made on the left and right sides of the top of the skull. CBB (CBB group) or CBB combined with bFGF (CBBbF group) were implanted on one side and non-implanted on the other side (control[Con] group). HBO group received HBO exposure for 2 weeks, and the rats were sacrificed at 4, 8 and 12 weeks after operation. The healing of bone defect was observed by radiographic and histological observation, and the expression of OPG and CD34 was evaluated by immunohistochemistry. Results The bone densities (BDs) of bone defect areas of the rats were significantly higher in the HBO-CBB, HBO-CBBbF, NHBO-CBB and NHBO-CBBbF groups than in the HBO-Con and NHBO-Con groups at 12 weeks after operation, and the HBO-CBBbF group has the highest BD (all P<0.01). The percentages of new bone formation areas were significantly higher in the HBO-CBB, HBO-CBBbF, NHBO-CBB and NHBO-CBBbF groups than in the HBO-Con and NHBO-Con groups (all P<0.01), and the blood vessel numbers were significantly larger in the HBO-CBBbF, HBO-CBB, NHBO-CBB and NHBO-CBBbF groups than in the HBO-Con and NHBO-Con groups at 12 weeks after operation. The blood vessel numbers were significantly larger in the HBO-CBBbF, HBO-CBB and NHBO-CBBbF groups than in the NHBO-CBB group at each time point after operation. The expression of OPG and CD34 was significantly higher in the HBO-CBBbF, HBO-CBB and NHBO-CBBbF groups than in the NHBO-CBB, HBO-Con and NHBO-Con groups (P<0.05, P<0.01). Conclusion CBB can promote bone defect repair and bFGF can increase bone regeneration and blood vessel number. HBO has synergistic effect on bFGF combined with CBB in repairing bone defects, and the effect is closely related to the promotion of OPG and CD34 expression. |
| Key words: hyperbaric oxygenation calcined bovine bone basic fibroblast growth factor bone regeneration CD34 antigen osteoprotegerin |
