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| 基于生物技术的肠道干细胞研究进展 |
| 冯珍兰1,2,许沁舒1,何翔1,杜继聪1,蔡建明1,2* |
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(1. 海军军医大学(第二军医大学)海军医学系, 上海 200433;
2. 温州医科大学公共卫生与管理学院, 温州 325000
*通信作者) |
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| 摘要: |
| 肠道干细胞对维持肠上皮各类细胞的稳态十分重要。正常情况下，位于隐窝基底部的肠道干细胞会不断向隐窝顶部迁移，在迁移过程中分化形成不同的肠上皮细胞。肠道干细胞大约每3～5 d更新1次，以维持肠道上皮的更新和损伤后修复。在肠道上皮完整的情况下，主要由活跃型肠道干细胞维持肠上皮的动态平衡；在肠道上皮损伤后，发挥修复作用的主要是静止型肠道干细胞。近年来，随着生物技术的发展，关于肠道干细胞的研究一直在不断进展。本文以国内外相关文献为基础，对应用于肠道干细胞研究中的主要生物技术、新发现的肠道干细胞亚群和标志物及调控因子进行综述。 |
| 关键词: 肠道干细胞 生物标志物 类器官 单细胞RNA测序 |
| DOI：10.16781/j.CN31-2187/R.20210578 |
| 投稿时间：2021-06-07修订日期：2021-08-26 |
| 基金项目:国家自然科学基金（81903260，81972978），上海市青年科技英才扬帆计划（19YF1459100），军队医学科技青年培育计划（21QNPY034）． |
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| Intestinal stem cells based on biotechnology:research progress |
| FENG Zhen-lan1,2,XU Qin-shu1,HE Xiang1,DU Ji-cong1,CAI Jian-ming1,2* |
(1. Faculty of Naval Medicine, Naval Medical University (Second Military Medical University), Shanghai 200433, China;
2. School of Public Health and Management, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
*Corresponding author) |
| Abstract: |
| Intestinal stem cells are very important for maintaining the homeostasis of various kinds of intestinal epithelial cells. Under normal circumstances, intestinal stem cells located at the base of the crypt will continue to migrate to the top of the crypt and differentiate into different intestinal mucosal cells. Intestinal stem cells are renewed every 3-5 d to maintain the renewal and repair of intestinal epithelium after injury. The dynamic balance of intestinal epithelium is mainly maintained by active intestinal stem cells when intestinal epithelium is intact, while the quiescent intestinal stem cells play a major role in repairing intestinal injury when intestinal epithelia is injuried. In recent years, with the development of biotechnology, the research on intestinal stem cells has been constantly updated and developed. Based on the relevant literatures at home and abroad, this paper reviews the main biotechnology, newly discovered intestinal stem cell subsets and markers and regulatory factors for intestinal stem cell research. |
| Key words: intestinal stem cells biomarkers organoid single cell RNA sequencing |
