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| 睑板腺囊肿患儿肠道菌群特征分析 |
| 崔元玥1,宋蕾1,李谐1,邱婷1,靳婧1,赵珂珂1,朱晓伟1,姜波2*,渠继芳1* |
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(1. 上海交通大学医学院附属上海儿童医学中心眼科, 上海 200127;
2. 安徽省第二人民医院眼科, 合肥 230041
*通信作者) |
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| 摘要: |
| 目的 初步分析睑板腺囊肿患儿肠道菌群的特征，并探讨肠道微生物组成与儿童睑板腺囊肿的关系。方法 收集21例睑板腺囊肿患儿和26名健康儿童的粪便样本，从粪便样本中提取出DNA，利用二代测序技术检测肠道菌群中的16S rRNA序列。通过测序结果分析睑板腺囊肿患儿和健康对照之间肠道微生物群组成。结果 通过α多样性及β多样性分析认为，健康对照组和睑板腺囊肿组肠道菌群多样性和相对丰度在组间及组内均无明显差异菌。通过线性判别分析效应大小（LEfSe）在两组的肠道菌群中筛选出11个差异物种。通过ANOSIM分析、t检验和metastats分析比较健康对照组和睑板腺囊肿组的肠道菌群，t检验结果发现7个差异物种，metastats分析发现40个差异物种，种水平上的gut_metagenome和human_gut_metagenome为共有差异物种。通过corrplot分析显示gut_metagenome与儿童睑板腺囊肿的个数、破溃及术后复发呈强相关性（r=0.70、0.76、0.85，P均<0.01）。结论 睑板腺囊肿患儿和健康儿童的肠道菌群多样性及相对丰度无明显差异，但某些菌种存在显著差异，gut_metagenome可能是一个独立于临床病理因素的、与睑板腺囊肿病情相关的微生物指标。 |
| 关键词: 睑板腺囊肿 胃肠道微生物组 测序 炎症 儿童 |
| DOI：10.16781/j.0258-879x.2021.12.1388 |
| 投稿时间：2021-08-05 |
| 基金项目:上海市科学技术委员会科研计划项目（19401932200），上海市卫生健康委员会科研项目（20194Y0268） |
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| Characteristics of intestinal flora in children with chalazion |
| CUI Yuan-yue1,SONG Lei1,LI Xie1,QIU Ting1,JIN Jing1,ZHAO Ke-ke1,ZHU Xiao-wei1,JIANG Bo2*,QU Ji-fang1* |
(1. Department of Ophthalmology, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China;
2. Department of Ophthalmology, the Second People's Hospital of Anhui Province, Hefei 230041, Anhui, China
*Corresponding authors) |
| Abstract: |
| Objective To analyze the characteristics of intestinal flora in children with chalazion, and explore the relationship between the composition of intestinal flora and chalazion.Methods Fecal samples were collected from 21 children with chalazion and 26 healthy children. DNA was extracted from the fecal samples, and the 16S rRNA sequences were detected by the second generation sequencing technology. The results were used to compare the composition of the microbiome between children with chalazion and healthy controls.Results There was no significant difference in intestinal flora diversity or relative abundance between or in the 2 groups according to the alpha and beta diversity. Eleven differential species were screened out by the linear discriminant analysis effect size (LEfSe) analysis. ANOSIM analysis, t test and metastats analysis were used to compare the intestinal flora of the healthy control group and chalazion group. The results of t test showed 7 differential species, and metastats analysis results showed 40 differential species. Gut_metagenome and human_gut_metagenome were common differential species at the species level. Finally, corrplot analysis showed that gut_metagenome was strongly correlated with the number, ulceration and postoperative recurrence of chalazion in children (r=0.70, 0.76, 0.85, all P < 0.01).Conclusion There is no significant difference in intestinal diversity or relative abundance between children with chalazion and healthy children, but there are significant differences in some bacterial species. Gut_metagenome may be a microbiological indicator which is independent of clinicopathologic factors, but is associated with chalazion disease. |
| Key words: chalazion gastro intestinal microbiome sequencing inflammation child |
