| 摘要: |
| 目的 探究海藻酸盐敷料联合负压封闭引流对大鼠海水浸泡创面的抗炎效果。方法 取10只健康雌性SD大鼠制备海水浸泡创面模型，随机分为两组（每组5只），实验组大鼠创面使用海藻酸盐敷料覆盖联合负压封闭引流装置，对照组使用常规急救敷料覆盖。造模后第5天取大鼠血液样本进行血常规检查。造模后第7天取大鼠创面组织，提取总RNA，采用qPCR分析TNF-α与IL-6 mRNA表达。造模后第7天取创面组织进行H-E和免疫组织化学染色，观察创面的组织学变化及创面组织中血管内皮生长因子（VEGF）的表达情况。结果 血常规检查结果显示，实验组大鼠白细胞计数低于对照组（P＜0.05）。qPCR检测结果显示，实验组大鼠创面组织中IL-6与TNF-α mRNA表达均低于对照组（P均＜0.01）。H-E染色结果显示，实验组大鼠创面组织中胶原纤维排列整齐，新生血管垂直于创面生长，创面可见明显上皮化；对照组上皮化不充分，肉芽组织内可见较多炎症细胞浸润。免疫组织化学染色结果显示，实验组创面组织中VEGF的平均光密度值高于对照组（P＜0.05）。结论 对于海水浸泡创面，海藻酸盐敷料联合负压封闭引流有利于减轻炎症反应，促进成纤维细胞增殖及微血管生成，从而促进伤口的良性愈合。 |
| 关键词: 海水浸泡 海藻酸盐敷料 负压封闭引流 炎症 创面 |
| DOI：10.16781/j.CN31-2187/R.20210408 |
| 投稿时间：2021-04-19 |
| 基金项目:军队医学科技青年培育计划项目（16QNP092） |
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| Anti-inflammatory effect of alginate dressing combined with vacuum sealing drainage on seawater immersion wounds |
| MEI Zi-jian1,JIANG Jia-lin1,ZHANG Wei-hang1,SONG Zhe-ming2*,GUO Yong-fei1* |
(1. The Second Ward, Department of Spinal Surgery, The Second Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200003, China;
2. Department of Anesthesiology, No. 905 Hospital of PLA Navy, Shanghai 200050, China
*Corresponding authors) |
| Abstract: |
| Objective To explore the anti-inflammatory effect of alginate dressing combined with vacuum sealing drainage on seawater immersion wounds in rats.Methods Ten healthy female SD rats were randomly divided into 2 groups, with 5 rats in each group. The wounds in the experimental group were covered with alginate dressing combined with vacuum sealing drainage, and the wounds in the control group were covered with conventional first aid dressing. Blood samples were taken for routine blood test on the 5th day after modeling. On the 7th day after modeling, total RNA was extracted from the wound tissues, and the mRNA expression of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) was analyzed by quantitative polymerase chain reaction (qPCR). Meanwhile, the wound tissues were taken for hematoxylin-eosin (H-E) staining and immunohistochemical staining to observe the histological change and the expression of vascular endothelial growth factor (VEGF).Results The results of routine blood test showed that the white blood cell count in the experimental group was significantly lower than that in the control group (P < 0.05). The results of qPCR showed that the mRNA expression levels of IL-6 and TNF-α in the experimental group were significantly lower than those in the control group (both P < 0.01). The results of H-E staining showed that the collagen fibers were arranged neatly, the neovascularization grew vertically, and the epithelialization was obvious in the wound tissues of rats in the experimental group; while the epithelialization was insufficient, and more inflammatory cells could be seen in the granulation tissues in the control group. The results of immunohistochemical staining showed that the average optical density of VEGF in the experimental group was significantly higher than that in the control group (P < 0.05).Conclusion For seawater immersion wounds, alginate dressing combined with vacuum sealing drainage can significantly reduce inflammatory reaction, promote proliferation of fibroblasts and angiogenesis, so as to promote wound healing. |
| Key words: seawater immersion alginate dressings vacuum sealing drainage inflammation wound |
