| 摘要: |
| 目的 观察四藤方对胃癌根治术后患者循环肿瘤细胞（CTC）数量的影响，探讨其解毒功效的现代细胞生物学机制。方法 选择2020年9月至2021年9月上海中医药大学附属龙华医院肿瘤科具有明确病理诊断的Ⅰ～Ⅱ期胃癌根治术后患者60例，按照随机数字表法分为四藤方组和对照组，每组30例。四藤方组予以四藤方＋中西医结合常规治疗，对照组予以中西医结合常规治疗。采集两组患者外周血，采用免疫磁珠阴性富集技术分离和富集CTC，结合荧光原位杂交法对CTC进行检测并计数，CTC≥1个/3.2 mL定义为阳性。结果 60例患者CTC检测阳性率为81.67%（49/60），其中对照组阳性率为90.00%（27/30），四藤方组阳性率为73.33%（22/30）。四藤方能降低早期胃癌根治术后患者CTC计数（P<0.01）。结论 四藤方能降低胃癌根治术后患者CTC数量，提示四藤方可能通过抑制CTC从而达到抗胃癌转移的作用。 |
| 关键词: 胃肿瘤 循环肿瘤细胞 四藤方 解毒机制 |
| DOI：10.16781/j.CN31-2187/R.20220524 |
| 投稿时间：2022-06-21修订日期：2022-12-04 |
| 基金项目:上海市临床重点专科建设项目（中医肿瘤）（SHSLCZDZK03701）. |
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| Detoxification mechanism of Siteng Fang based on circulating tumor cells in postoperative gastric cancer patients |
| MAO Zhu-jun,LIU Ke-xian,SHEN Ke-ping* |
(Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
*Corresponding author) |
| Abstract: |
| Objective To observe the effect of Siteng Fang on the count of circulating tumor cells (CTCs) in patients with gastric cancer, and to explore the modern cell biological mechanism of its detoxification effect. Methods From Sep. 2020 to Sep. 2021, 60 patients of stageⅠ-Ⅱ gastric cancer after radical surgery with clear pathological diagnosis in Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine were randomly divided into Siteng Fang group and control group according to the random number table method, with 30 cases in each group. The Siteng Fang group was treated by Siteng Fang+conventional treatment with traditional Chinese and Western medicine, and the control group was treated by conventional treatment with traditional Chinese and Western medicine. Peripheral blood was collected from both groups, and CTCs were isolated and enriched by immunomagnetic bead negative enrichment technique, combined with fluorescence in situ hybridization to detect and count CTCs, and CTCs ≥ 1/3.2 mL was defined as positive. Results The positive rate of CTC detection was 81.67% (49/60), including 90.00% (27/30) in the control group and 73.33% (22/30) in the Siteng Fang group. Siteng Fang reduced the CTC counts in patients after radical surgery of early gastric cancer (P<0.01). Conclusion Siteng Fang can reduce the number of CTCs in patients after radical surgery of gastric cancer, suggesting that Siteng Fang may have anti-metastasis effect on gastric cancer by inhibiting CTCs. |
| Key words: stomach neoplasms circulating tumor cells Siteng Fang detoxification mechanism |
