| 摘要: |
| 目的 观察芦丁对减压病（DCS）大鼠模型的保护作用。方法 将80只大鼠随机分为正常对照组（n=12）、DCS模型组（n=34）、芦丁＋DCS模型组（n=34）。采用空气加压模拟潜水方法建立大鼠DCS模型，比较3组大鼠的DCS发病率、发病起始时间、脊髓组织病理改变、脊髓组织中炎症因子和氧化应激指标含量变化、肺组织病理病变、肺湿/干比及肺泡灌洗液蛋白质含量等指标，评估芦丁处理对大鼠DCS损伤的作用。结果 空气加压模拟潜水后DCS模型组大鼠DCS发病率为47.06%（16/34），芦丁＋DCS模型组大鼠DCS的发病率［26.47%（9/34）］较DCS模型组降低，但差异无统计学意义（P=0.078）。芦丁＋DCS模型组大鼠DCS发病起始时间为（9.53±1.88）min，较DCS模型组的发病起始时间［（6.27±2.64）min］延迟，差异有统计学意义（P<0.05）。与正常对照组相比，DCS模型组中大鼠脊髓组织TNF-α、IL-1β、丙二醛含量升高，肺泡灌洗液蛋白质含量、肺湿/干比升高，差异均有统计学意义（P均<0.05）；与DCS模型组比较，芦丁＋DCS模型组大鼠上述指标均降低（P均<0.05）。芦丁处理还能减轻DCS模型大鼠肺组织病理改变。结论 芦丁处理能够减轻大鼠DCS损伤，对模型大鼠具有较好的保护作用。 |
| 关键词: 减压病 芦丁 脊髓损伤 肺损伤 炎症 氧化性应激 |
| DOI：10.16781/j.CN31-2187/R.20220881 |
| 投稿时间：2022-11-17修订日期：2022-12-20 |
| 基金项目:国家自然科学基金（81801868）. |
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| Protective effect of rutin on a rat model of decompression sickness |
| DING Jie1△,ZHANG Ya-nan2△,MENG Xiang-yang2,ZHENG Juan2,HUANG Guo-yang2* |
(1. Department of Emergency, The First Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200433, China;
2. Department of Diving and Hyperbaric Medical Research, Naval Medical Center, Naval Medical University (Second Military Medical University), Shanghai 200433, China
△Co-first authors.
* Corresponding author) |
| Abstract: |
| Objective To observe the protective effect of rutin on a rat model of decompression sickness (DCS). Methods Eighty rats were randomly divided into normal control group (n=12), DCS group (n=34), and rutin+DCS group (n=34). An air-pressurized simulated diving method was used to establish a rat DCS model. The incidence and onset time of DCS, pathological changes of spinal cord and lung tissues, content of inflammation factors and oxidative stress index in spinal cord tissues, lung wet/dry ratio and protein content in alveolar lavage fluid were compared among the 3 groups to evaluate the effect of rutin on DCS injury in rats. Results The incidence of DCS in DCS group was 47.06% (16/34), the incidence of DCS in rutin+DCS group (26.47%, 9/34) was decreased compared with DCS group, but with no statistical difference (P=0.078). The DCS onset time in rutin+DCS group was (9.53±1.88) min, which was significantly delayed compared with DCS group ([6.27±2.64]min) (P<0.05). Compared with normal control group, the contents of tumor necrosis factor α, interleukin 1β and malondialdehyde in spinal cord tissues, the protein content in alveolar lavage fluid, and lung wet/dry ratio in DCS group were significantly increased (all P<0.05); and compared with DCS group, the above indexes were decreased in rutin+DCS group (all P<0.05). Rutin treatment also alleviated the pathological changes of lung tissues in DCS model rats. Conclusion Rutin treatment can improve DCS injuries in rats, and has a good protective effect on model rats. |
| Key words: decompression sickness rutin spinal cord injury lung injury inflammation oxidative stress |
