| 摘要: |
| 目的 制备黄连素-白及多糖-羧甲基壳聚糖复合敷料（BBCCD），并评估其物理性能及药效。方法 采用真空冷冻干燥法制备BBCCD。根据孔隙率、吸水率、透气率筛选最佳处方配比。通过扫描电子显微镜观察所制备的BBCCD的微观结构，抑菌圈法评价其抑菌效果，CCK-8法检测其细胞毒性。将新西兰兔血液样本随机分为医用纱布组、明胶海绵组、BBCCD组，通过体外凝血实验评价BBCCD的凝血效果（n＝6）。将72只SD大鼠随机分为医用纱布组、明胶海绵组和BBCCD组，通过大鼠股静脉、肝脏及断尾止血实验分析止血时间（n＝8）；将54只SD大鼠随机分为空白组、壳聚糖敷料组和BBCCD组，采用伤口愈合率（n＝6）和血清IL-1β、TNF-α、IL-10水平（n＝4）测定及创面病理学变化综合评价BBCCD的促愈合效果。结果 BBCCD中5%白及多糖溶液与5%羧甲基壳聚糖溶液的最佳体积比为1∶3。BBCCD为膜性海绵状多孔结构，无明显毒性，可吸收创面渗液而产生强黏附性，黄连素含量为（52.39±0.87）mg/g，对金黄色葡萄球菌和大肠埃希菌均有抑菌效果。BBCCD组的体外凝血指数、股静脉创伤止血时间、肝脏创伤止血时间、断尾止血时间均优于明胶海绵组和医用纱布组（P均＜0.01）。在皮肤创面修复的第3、5、7、14天，BBCCD组大鼠的伤口愈合率均高于壳聚糖敷料组和空白组（P均＜0.05）。在皮肤创面修复的第7、14天，与壳聚糖敷料组和空白组相比，BBCCD组大鼠血清IL-10水平均升高，IL-1β、TNF-α水平均降低（P均＜0.05）。病理结果显示，BBCCD在伤口愈合的初期可促进新生肉芽组织和毛细血管的生成，减少炎症反应，加速创面愈合。结论 所制备的BBCCD性质稳定，具有良好的抑菌、止血及促创面愈合效果。 |
| 关键词: 黄连素 白及多糖 羧甲基壳聚糖 复合敷料 止血 创面愈合 |
| DOI：10.16781/j.CN31-2187/R.20220930 |
| 投稿时间：2022-12-13修订日期：2023-02-23 |
| 基金项目:海军军医大学（第二军医大学）第一附属医院“深蓝123”项目（2019SLZ015）. |
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| Design and pharmacodynamic evaluation of berberine-Bletilla striata polysaccharide-carboxymethyl chitosan composite dressing |
| GONG Yan-rong1△,WANG Zhi-bo2△,SHI Lei2,WANG Yu-qing2,XIANG Xing1,ZHANG Hui-qing1* |
(1. Department of Traditional Chinese Formulaology, School of Traditional Chinese Medicine, Naval Medical University(Second Military Medical University), Shanghai 200433, China;
2. College of Basic Medical Sciences, Naval Medical University(Second Military Medical University), Shanghai 200433, China
△Co-first authors.
* Corresponding author) |
| Abstract: |
| Objective To prepare the berberine-Bletilla striata polysaccharide-carboxymethyl chitosan composite dressing (BBCCD) and evaluate its physical properties and efficacy. Methods The BBCCD was prepared by vacuum freeze-drying method. The optimum proportion was selected based on porosity, water absorption and air permeability. The microstructure of BBCCD was observed by scanning electron microscopy, the antibacterial activity was evaluated by antibacterial experiment, and the cytotoxicity was tested by cell counting kit 8 method. The blood samples of New Zealand rabbits were randomly divided into gauze group, gelatin sponge group and BBCCD group, and the coagulation effect of BBCCD was evaluated by in vitro coagulation experiment (n＝6). Seventy-two SD rats were randomly divided into gauze group, gelatin sponge group and BBCCD group, and the hemostatic time was evaluated by femoral vein, liver and tail-cut hemostasis experiments (n＝8). Fifty-four SD rats were randomly divided into control group, chitosan group and BBCCD group, and the wound repair effects of BBCCD were evaluated by wound healing rate (n＝6), detection of serum interleukin (IL)-1β, tumor necrosis factor α (TNF-α) and IL-10 (n＝4) and histopathological examination. Results The optimal volume ratio of 5% Bletilla striata polysaccharide to 5% carboxymethyl chitosan composite in BBCCD was 1∶3. BBCCD had a membranous sponge-like porous structure with no obvious toxicity. It could absorb wound exudate and had strong adhesion. The content of berberine was (52.39±0.87) mg/g. BBCCD had antibacterial effects on Staphylococcus aureus and Escherichia coli. The in vitro blood clotting index and the hemostatic time of femoral vein trauma, liver trauma and tail-cut in the BBCCD group were significantly better than those in the gelatin sponge group and gauze group (all P＜0.01). On the 3rd, 5th, 7th and 14th days, the wound healing rate of the BBCCD group was significantly higher than that of the chitosan group and control group (all P＜0.05). On the 7th and 14th days, the serum level of IL-10 in the BBCCD group was significantly increased and the serum levels of IL-1β and TNF-α were significantly decreased compared with the chitosan group and control group (all P＜0.05). The pathological results showed that BBCCD could promote the formation of granulation tissue and capillaries at the initial stage of wound healing, attenuate inflammatory response, and accelerate wound healing. Conclusion The prepared BBCCD has excellent antibacterial activity, hemostasis and wound healing effects with stable properties. |
| Key words: berberine Bletilla striata polysaccharide carboxymethyl chitosan composite dressing hemostasis wound healing |
