| 摘要: |
| 单细胞转录组测序联合空间转录组测序可解析组织内每一个细胞的转录组表达谱和空间位置信息，有助于探究细胞的基因表达水平和组织的三维重建，解读细胞异质性，理解细胞间的联系和相互作用，对于深入探讨疾病发生和发展的细胞与分子机制具有重要意义。纤维化疾病累及全身多个器官，病程极为复杂，缺乏有效的抗纤维化治疗方法，因此仍需加强对此类疾病的全面认识。近年来，单细胞转录组测序联合空间转录组测序已应用于纤维化疾病的病理生理机制研究中，取得了较多进展。本文简要介绍单细胞转录组测序联合空间转录组测序技术，并对其在纤维化疾病研究中的应用进展作一综述。 |
| 关键词: 转录组学 单细胞转录组测序 空间转录组测序 纤维化 纤维化疾病 |
| DOI：10.16781/j.CN31-2187/R.20230181 |
| 投稿时间：2023-04-07修订日期：2023-06-20 |
| 基金项目:国家自然科学基金面上项目（81972397），上海市青年科技启明星计划（22QA1411500）. |
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| Single-cell transcriptom sequencing combined with spatial transcriptom sequencing in fibrotic diseases: research progress |
| HU Yifan1,HU Jinghan1,JIANG Junfeng2,XIONG Jun2* |
(1. Student Team, College of Basic Medical Sciences, Naval Medical University(Second Military Medical University), Shanghai 200433, China;
2. Department of Histology and Embryology, College of Basic Medical Sciences, Naval Medical University(Second Military Medical University), Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| The combination of single-cell transcriptome sequencing and spatial transcriptome sequencing can analyze the transcriptome and spatial location information of each cell within the tissue, which helps to explore the gene expression level of cells and the 3-dimensional reconstruction of tissue, decipher cell heterogeneity, understand the connections and interactions between cells, and deeply explore the cellular and molecular mechanisms of disease development and progression. Fibrotic diseases involve multiple organs, the course of the disease is extremely complex, and there is a lack of effective anti-fibrotic treatment; it is still necessary to strengthen the comprehensive understanding of the disease. In recent years, single-cell transcriptome sequencing combined with spatial transcriptome sequencing has been applied for the research on pathophysiological mechanisms of fibrotic diseases, and more progress has been made. This article briefly introduces single-cell transcriptome sequencing combined with spatial transcriptome sequencing and reviews its research progress in fibrotic diseases. |
| Key words: transcriptomics single-cell transcriptome sequencing spatial transcriptome sequencing fibrosis fibrotic disease |
