| 本文已被：浏览 67次 下载 19次 |
码上扫一扫！ |
| 基于16S rRNA高通量测序的驻岛人员肠道菌群分析 |
| 吴浩1,谢沛1,张菂1,黄文1,李兆申1,2,薛芊2*,鞠金涛3* |
|
|
(1. 海军军医大学(第二军医大学)第一附属医院消化内科, 上海 200433;
2. 海军军医大学(第二军医大学)第一附属医院临床研究中心, 上海 200433;
3. 海军后勤保障部卫生局, 北京 100841
*通信作者) |
|
| 摘要: |
| 目的 分析驻岛人员肠道菌群特征，为维护驻岛人员的肠道微生态平衡及提供精准的医疗保障奠定基础。方法 采用随机抽样的方法纳入驻岛人员若干，同时纳入沿海地区人员若干作为对照，对人员粪便标本进行16S rRNA高通量测序。比较两组人员肠道菌群多样性及菌群组成差异。结果 肠道菌群alpha多样性分析显示驻岛人员肠道菌群丰度较沿海地区人员升高，beta多样性分析提示驻岛人员肠道微生物群落构成与沿海地区人员相比差异有统计学意义（P＝0.001）。驻岛人员肠道内拟杆菌门丰度低于沿海地区人员（30.8% vs 48.3%，P＜0.001），而肠道内变形菌门丰度高于沿海地区人员（28.3% vs 10.2%，P＜0.001）。经多重假设检验校正后发现，驻岛人员肠道内拟杆菌属、罗斯氏菌属、另枝菌属、副拟杆菌属丰度显著下降，普雷沃氏菌属、大肠埃希菌-志贺菌属、柠檬酸杆菌属、产粪甾醇真杆菌属丰度显著提升。结论 海岛特殊环境影响驻岛人员肠道菌群特征，肠道微生态健康亟需精准保障。 |
| 关键词: 驻岛人员 海岛 肠道菌群 16S rRNA 高通量测序 |
| DOI：10.16781/j.CN31-2187/R.20240593 |
| 投稿时间：2024-08-26修订日期：2024-12-17 |
| 基金项目: |
|
| Intestinal flora of personnel stationed on an island based on 16S rRNA high-throughput sequencing |
| WU Hao1,XIE Pei1,ZHANG Di1,HUANG Wen1,LI Zhaoshen1,2,XUE Qian2*,JU Jintao3* |
(1. Department of Gastroenterology, The First Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200433, China;
2. Clinical Research Unit, The First Affiliated Hospital of Naval Medical University (Second Military Medical University), Shanghai 200433, China;
3. Health Bureau, Logistics Department of PLA Navy, Beijing 100841, China
*Corresponding authors) |
| Abstract: |
| Objective To analyze the characteristics of intestinal flora of personnel stationed on an island, so as to lay the foundation for maintaining the intestinal microecological balance of personnel stationed on island and provide accurate medical security. Methods Several subjects stationed on an island and several subjects from coastal areas were enrolled by random and sampling method, and their fecal samples were sequenced by 16S rRNA high-throughput sequencing. Diversity and composition of gut microbiota in 2 cohorts of personnel were compared. Results Alpha diversity analysis of intestinal flora showed that the abundance of intestinal flora in subjects stationed on the island was significantly higher than that of subjects from coastal areas. Beta diversity analysis indicated significant differences in the composition of intestinal microbial communities between the subjects stationed on the island and those from coastal areas (P＝0.001). The abundance of the Bacteroidota in the intestinal tract of subjects stationed on the island was significantly lower than that of subjects from coastal areas (30.8% vs 48.3%, P＜0.001), while the abundance of the Proteobacteria was significantly higher than that of subjects from coastal areas (28.3% vs 10.2%, P＜0.001). After multiple hypothesis testing correction, it was found that the abundance of the Bacteroides, Roseburia, Alistipes, and Parabacteroides in the intestines of subjects stationed on the island decreased significantly, while the abundance of the Prevotella, Escherichia-Shigella, Citrobacter, and Eubacterium_coprostanoligenes increased significantly. Conclusion The special environment of islands affects the characteristics of intestinal flora of personnel, and the intestinal microecological health needs precise maintenance. |
| Key words: personnel stationed on an island islands intestinal flora 16S rRNA high-throughput sequencing |
