| 摘要: |
| 目的：探讨B细胞与人肝癌细胞的融合细胞经60Co照射后的生长和免疫原性相关表型的变化。方法：体外照射后观察融合细胞生长曲线和软琼脂集落形成；体内采用裸鼠成瘤试验，观察体外生长和动物体内成瘤性，应用流式细胞仪检测照射后融合细胞MHC-Ⅰ，MHC-Ⅱ，B7及肝癌抗原(GP75)表达，观察其免疫原性相关表型变化。结果：经60Co照射的融合细胞失去增殖能力，4 d后可见部分细胞死亡，7 d后几乎全部死亡。60Co照射后3 d内MHC-Ⅰ，MHC-Ⅱ，B7及GP75表达无明显变化。结论：经60Co照射后融合细胞失去增殖能力，但3 d内免疫相关表型无明显变化。 |
| 关键词: 肝肿瘤 疫苗 辐射 细胞融合 |
| DOI： |
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| 基金项目: |
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| Observation of growth and phenotype of 60Co radiated B-cell-human hepatoma cell fusion vaccine |
| 刘彦君,王皓,阎振林,钱卫珠,卫立辛 |
| () |
| Abstract: |
| Objective: To investigate the growth and the immunogenicity-related phenotype of B cell-human hepatoma cell fusion cells after 60Co-radiation. Methods: Growth curve and soft agar colonogenicity of fusion tumor vaccine in vitro, and their tumorigenicity in nude mice in vivo for the radiated cell fusion tumor vaccine were observed. The expression of MHC-Ⅰ,MHC-Ⅱ, B7 and hepatoma-antigen(GP75) that were related to immunogenicity of the radiated fusion tumor vaccine cell were analyzed by flow cytometry. Results: 60Co-radiated fusion cells lost proliferative capacity, partly died on d 4 and almost completely died on d 7,while MHC-Ⅰ,MHC-Ⅱ, B7 and GP75 had no apparent changes in immunogenicity-related phenotype in the first 3 d. Conclusion: 60Co-radiated fusion cells lose proliferative capacity, but they have no apparent changes in immunogenicity-related phenotype in the first 3 d. |
| Key words: hepatoma vaccine radiation cell fusion |
