| 摘要: |
| 目的：提供简便有效的骨髓细胞(BMC)保存方法，以便进一步提高骨髓移植效果。方法：以昆明种小鼠急性放射病为模型，进行了-80℃冷冻保存BMC及其程序移植的研究。结果：-80℃保存的BMC其有核细胞回收率为(89.0±3.5)％，锥虫蓝拒染率为(85.0±3.6)％；产率(每105个BMC)：单核巨噬细胞克隆形成单位(CFU-GM)为108±10，脾细胞克隆形成单位(CFU-S)为12±3。昆明种小鼠程序移植-80℃保存的同种异基因BMC 4×105，其活存率与一次移植同条件冻存的BMC 1×107相同(30％)；程序移植4×106时，活存率显著提高(60％)。经性染色体检查各组均有植入证据。结论：-80℃保存BMC是一种行之有效的方法；骨髓程序移植可能充分利用了每次腾出的“龛位”，减少了移植细胞数，却增加了干细胞植入，提高了移植效果，是一种“种子与土壤”并重的移植新方法。 |
| 关键词: 骨髓移植 龛位 冷冻保存 辐射损伤 |
| DOI： |
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| 基金项目: |
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| Experimental sutdy on -80℃ cryopreservation of bone marrow cells and bone marrow scheduled transplantation |
| 张明华,丁振海,杨平,杨如俊,项莺松 |
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| Abstract: |
| Objective: To enhance the efficiency of bone marrow transplantation (BMT) and to provide a simple and effective method for cryopreservation of bone marrow cells (BMCs). Methods: BMCs were cryopreservated at -80℃ and a new method for bone marrow scheduled transplantation (BMST) was studied with acute radiation disease model of Kunming mice. Results: The method for cryopreservation of BMCs at -80℃ produced the highest recovery rates for nucleated cells (89.0%±3.5%), the trypan blue exclusion (85.0%±3.6%), CFU-GM ［(108±10)/105 BMC］, CFU-S［(12±3)/105 BMC］. Using this method, lethally irradiated Kunming mice received allogenetic BMST. The survival rate of mice (30%) in BMST group transplanted with 4×105 BMCs was the same as that in once BMT group transplanted with 1×107 BMCs. And the survival rate of mice (60%) in BMST transplanted with 4×106 BMCs was significantly higher than that in once BMT. Evidence of engraftment could be detected in all groups. Conclusion: This method for cryopreservation BMCs at -80℃ is simple and effective, and these data suggest that “Niches” vacated each time could be fully used in BMST, so the transplanted BMCs could be decreased, the engraftment of stem cells could be increased, and the grafting efficiency could be enhanced. This method is a new method aiming at “both seed and soil”. |
| Key words: bone marrow transplantation niche freeze preservation radiation injuries |
