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白细胞介素6、肿瘤坏死因子对体外培养的血管内皮细胞的损伤作用
丁思奇,吴宗贵,侯健,张国元,马丽萍
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摘要:
目的:研究白细胞介素6(IL-6)和肿瘤坏死因子(TNF)对人脐静脉内皮细胞(HVEC)的损伤作用。方法:以原代培养的HVEC为模型,将IL-6、TNF及其抗体与HVEC共同孵育,观察内皮细胞(EC)形态变化和培养液中血管紧张素转化酶(ACE)和乳酸脱氢酶(LDH)水平。结果: TNF和IL-6可引起细胞间隙增大,甚至细胞脱落;并且随着TNF和IL-6浓度加大及作用时间延长,培养液中ACE和LDH水平升高。结论: IL-6和TNF对血管EC有损伤作用。
关键词:  内皮,血管  肿瘤坏死因子  白细胞介素6
DOI:
基金项目:
Effects of interleukin-6 and tumor necrosis factor on cultured vascular endothelial cells
丁思奇,吴宗贵,侯健,张国元,马丽萍
()
Abstract:
Objective: To study the damaging effects of interleukin-6 (IL-6) and tumor necrosis factor (TNF) on human vascular endothelial cells (HVEC). Methods: A HVEC model was developed. After being incubated with IL-6, TNF and their antibody, HVEC morphological changes were observed, and the levels of lactate dehydrogenase (LDH) and angiotensin converting enzyme (ACE) in the culture fuild were measured. Results: TNF and IL-6 could cause enlargement in intercellular space, even the exfoliation of HVEC, moreover, ACE and LDH levels increased in a time- and dose-dependent manner. Conclusion: IL-6 and TNF have damaging effects on HVEC.
Key words:  endothelium, vascular  tumor necrosis factor  interleukin-6