| 摘要: |
| 目的：观察去甲斑蝥素(NCTD)体外对卵巢癌3AO细胞和AO细胞的抑制作用，并初步探讨其作用机制。方法：利用细胞培养技术，以结晶紫染色法测定NCTD对卵巢癌3AO和AO细胞的抑制作用，并利用流式细胞仪测定分析NCTD作用下两种肿瘤细胞的细胞周期变化。结果：NCTD 1μg/ml即对3AO和AO细胞有轻微抑制作用，其半数抑制剂量(IC50)分别为20μg/ml和10μg/ml。NCTD作用6h，3AO和AO细胞生长即受到抑制，其抑制率随着药物作用时间延长而升高。在NCTD作用下，3AO和AO细胞G2+M期细胞百分比明显升高，而S期细胞百分比相应地明显降低。结论：NCTD体外对卵巢癌3AO和AO细胞有明显抑制作用，其抑制作用呈剂量和时间依赖性。NCTD抗肿瘤细胞增殖的机制可能与其将肿瘤细胞特异阻抑于G2+M期有关。 |
| 关键词: 去甲斑蝥素 卵巢肿瘤 细胞周期 |
| DOI： |
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| In vitro inhibitory effect of norcantharidine on ovarian carcinoma 3AO and AO cells |
| 曹颖瑛,朱玲仙,宓鹤鸣,郑钦岳,王昭梅,唐龙英 |
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| Abstract: |
| Objective:To evaluate the inhibitory effects of norcantharidine(NCTD) on ovarian carcinoma 3AO and AO cells cultured in vitro and to reveal its mechanism.Methods:Using cell culture technique,the inhibitory effects of NCTD on 3AO and AO cells were measured by way of crystal violet dyeing.The alterations of cell cycle induced by NCTD were analyzed with flow cytometry.Results:3AO and AO cells exposed to NCTD 1 μg/ml were inhibited slightly.The IC50 values of NCTD for them were 20 μg/ml and 10 μg/ml respectively.The growth of 3AO and AO cells was inhibited after being cultured with NCTD for 6 h. The inhibitory rates were increased as time of culture prolonged.The percent age of 3AO and AO cells in G2+M phase were increased obviously and the percentage of cells in S phase were decreased accordingly after treated with NCTD. Conclusion: NCTD has a significant inhibition of ovarian carcinoma 3AO and AO cells,which is in a dose- and time-dependent manner. The mechanism of NCTD-induced antiproliferative effect appears to involve blockade of tumor cells in the G2+M phase of the cell cycle. |
| Key words: norcantharidine ovarian neoplasms cell cycle |
