| 摘要: |
| 目的：研究不同剂量的普鲁泊福对犬急性缺血再灌注损伤心肌代谢和超微结构的影响。方法：杂种犬18只（11-14kg），随机分3组：生理盐水对照组(NS组)用量为2ml/(kg.h), 高剂量普鲁泊福组(HP组)用量为11.2mg/(kg.h), 低剂量普鲁泊福组(LP组)用量为5.6mg/(kg.h)。开胸阻断左冠状动脉前降支（LAD）90min后再灌注200 min;于注药前、注药60min、阻断LAD血流90min以及再灌注60,120和180min时，抽心大静脉血测乳酸脱氢酶(LDH)含量和超氧化物歧化酶(SOD)活力；于再灌注200min时取再灌注区心肌组织做电镜观察。结果：再灌注后各时间点NS组LDH含量明显高于HP组和LP组（P<0.05），SOD活力明显低于HP组和LP组（P<0.05）。NS组心肌超微结构再灌注损伤明显重于HP组和LP组。结论：普鲁泊福具有一定抗氧化能力和保护线粒体作用，可减轻犬急性心肌缺血再灌注损伤。 |
| 关键词: 普鲁泊福、再灌注损伤、乳酸脱氢酶 超氧化物歧化酶 心肌 |
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| Effect of propofol on metabolism and ultrastructure of acute ischemia-reperfusion myocardium in a canine model |
| 刘树孝,徐美英,邓小明,周勇,王粤,朱科明 |
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| Abstract: |
| Objective: To evaluate the effects of propofol on metabolism and ultrastructure of acute ischemia-reperfusion myocardium in an open-chest canine model. Methods: Eighteen hybrid dogs(11-14kg) were divided randomly into 3 groups: NS group (normal saline), 2ml/(kg.h)； HP group (high-dose propofol), 11.2 mg/(kg.h)；LP group (low-dose propofol), 5.6mg/(kg.h). These dogs were subjected to a 90min left anterior descending coronary artery (LAD) occlusion followed by 200 min of reperfusion. Before administration, 60min after administration, 90min after occlusion and 60, 120,180 min after reperfusion, lactate dehydrogenase(LDH) and superoxide dismutase(SOD) in great cardiac vein were detected. 200min after reperfusion myocardial ultrastructure was examined by electronic-microscope. Results: Compared with the value in HP and LP group, LDH in NS group was significantly higher, SOD was significantly lower(P<0.05) during reperfusion. Impairment of myocardial ultrastructure was most severe in NS group. Conclusion: Propofol can reduce myocardial ischemia-reperfusion injury in a canine model with its anti-oxidation and protection of chondriosome. |
| Key words: propofol reperfusion injury lactate dehydrogenase superoxide dismutase myocardium |
