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葛根素对培养人主动脉内皮细胞脂质过氧化的保护作用
秦永文,沈茜,章同华,丁继军
0
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摘要:
目的:观察葛根素对培养人主动脉内皮细胞脂质过氧化的保护作用。方法:建立人主动脉内皮细胞体外培养模型,在培养液中加入0.1×10-4和1.0×10-4mol/L联胺,致内皮细胞脂质过氧化损害,即刻或30min后加入葛根素0.3mg/孔,测定噻唑蓝摄取率、乳酸脱氢酶(LDH)、丙二醛(MDA)和凝血?蓖?TXA2)含量。结果:两种浓度的联胺使LDH,MDA和TXA2含量增加,使噻唑蓝摄取率下降,加入葛根素后可使上述结果逆转。结论:葛根素对脂质过氧化致内皮细胞的损害具保护作用。
关键词:  内皮细胞、联胺、葛根素  脂质过氧化
DOI:
基金项目:
Protective effect of puerarin on cultured human aortic endothelial cells injury caused by lipid peroxide
秦永文,沈茜,章同华,丁继军
()
Abstract:
Objective: To observe the protective effect of puerarin on cultured human aortic endothelial cells (ECs) injury caused by lipid peroxide. Methods:ECs were treated with diamide to stimulate and faciliate lipid peroxidation. The diamide concentration was 0.1×10-4 and 1.0×10-4mol/L. Then 0.3mg puerarin was added immediately or 0.5h later. Results: Puerarin decreased the LPO and TXA2, then the MTT increased and LDH release decreased. Conclusion: Puerarin reduces the injury of ECs caused by lipid peroxide.It might play an important role in antiatherongenesis.
Key words:  endothelial cell  diamide  puerarin  lipid peroxidation