| 摘要: |
| 目的：观察促性腺激素释放激素(LHRH)高效类似物那法瑞林对人乳腺癌BCaP-37细胞裸鼠移植瘤生长的抑制作用并探讨其作用机制。方法：那法瑞林用药分为移植瘤接种后次日给药组及接种后2周给药组，每组各设3个剂量，均连续皮下给药4周。使用他莫昔芬和另一LHRH激动剂D-Trp6 LHRH作为阳性对照。用药结束后，分别测定移植瘤、双侧卵巢、子宫质量和血清雌二醇(E2)含量；进行病理检查(包括电镜)；测定移植瘤细胞DNA指数(DI)；观察瘤细胞增殖周期比例的变化。结果：(1)在以上不同的两用药组中，那法瑞林剂量在500和1000μg/kg时，可以显著抑制移植瘤的生长，移植瘤的DI值明显下降，通过电镜观察到瘤组织内存在凋亡的瘤细胞。同时，动物的卵巢质量和血清E2含量显著下降。(2)他莫昔芬30mg/kg对移植瘤生长无显著影响，与D-Trp6 LHRH 抑制移植瘤生长的作用相比亦无显著差别。结论：那法瑞林长期皮下给药对BCaP-37移植瘤生长有显著的抑制作用。 |
| 关键词: 促性腺激素释放激素 乳腺肿瘤 那法瑞林 |
| DOI： |
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| Inhibitory effect of nafarelin on the growth of BCaP-37 human breast cancer cell xenografts in nude mice |
| 褚云宏,周颖奇,顾军 |
| () |
| Abstract: |
| Objective: To study the effect and mechanism of LHRH analogue nafarelin on the growth of BCaP-37 human breast cancer cell xenografts in nude mice. Methods: Nafarelin treatment began at next day or 2 weeks after tumor inoculation and each group had 3 different doses by s.c. per day for 4 weeks. Tamoxifen and another LHRH analogue D-Trp6 LHRH were used as positive control. The changes of xenografted tumor induced by nafarelin treatment were investigated with respect to its histology, cell DNA index (DI), FCM, serum level of E2 as well as the mass of tumor, uterus and bilateral ovaries. Results: (1) Chronic administration of nafarelin at doses of 500 or 1 000 μg/kg resulted in a significant inhibition of tumor growth and a decrease in DI and serum level of E2, apoptosis of tumor cells in nafarelin treated groups was also observed. (2) Tamoxifen had no inhibitory effect on the growth of xenografted tumor at 30 mg/kg. D-Trp6 LHRH had a potency comparable to nafarelin in suppressing the growth of BCaP-37 xenografted tumor. Conclusion: Chronic administration of nafarelin could inhibit the growth of xenografted BCaP-37 breast cancer significantly. |
| Key words: gonadorelin breast neoplasms nafarelin |
