| 摘要: |
| 目的：观察雷公藤红素诱导人肥大细 胞系(HMC-1)细胞凋亡过程中细胞形态的变化，并探讨其意义。方法：在HMC-1细胞培养体系中加入雷公藤红素，用光镜、电镜观察细胞的形态变化，用TUNE L和流式细胞术(FCM)检测细胞凋亡。结果：细胞在雷公藤红素作用下呈葵 样改变：细胞母体居中，周边围以形成或正在形成的凋亡小体。葵样细胞（sunflower-like cell, SLC）对锥虫蓝拒染，可伴有核染色质浓缩和着边，药物作用20 min即可形成，持续数小时后完全裂解为凋亡小体，或凋亡小体游离,残留细胞母体，最后崩溃；SLC的形成具 有时间和药物浓度依赖性；在8 h内与TUNEL标记和碘化丙啶染色/FCM检测的凋亡结果有显著 相关性；SLC清晰易辨，个体内和个体间计数重复性良好。结论：SLC 是凋亡细胞的特征性形态改变，可作为检测HMC-1细胞凋亡的定性和定量指标。 |
| 关键词: 肥大细胞 凋亡 葵样细胞 |
| DOI： |
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| Sunflower-like cell: characteristic morphologic changes of apoptosis |
| 张登海,张玲珍,李莉,叶挺军,孔宪涛,鲍一笑 |
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| Abstract: |
| Objective: Apoptosis of human mast cell line (HMC-1) could be i nduced by tripterine, one of the pure ingredients of tripterygium wilfordii hook . The paper is to explore the cell changes in morphology of apoptotic cells. Methods: On or after exposure to tripterine, cells were observed with microscopy and electromicroscopy. Apoptosis was detected by flowcytometry and T UNEL. Results: Sunflower-like cell (SLC), cell surrounded by formed or forming apoptotic bodies with chromatin condensed or not, were seen in the apoptotic process, which was negtive to trypan blue dye. SLC could occur in 20 minutes after incubated with tripterine and survive for several hours. The f ormation of SLC was at a time- and dose- dependent manner. The results of apop tosis assayed by SLC count were significantly correlated with that mearsured by TUN EL or PI/FCM in first 8 h. The SLC could easily be determined by microscopy in t he condition of culture. The results of SLC counted by inter- or intra-examine r were consistent. Conclusion: The results suggest that SLC is a characteristic morphologic change of apoptotic cells, and can be a good indicat or for HMC-1 cell apoptosis. |
| Key words: mast cells apoptosis sunflower-like cell |
