| 摘要: |
| 目的：克隆大鼠肝脏再生增强因子（ALR）cDNA，并对其序列进行分析。方法：建立大鼠2/3肝切除模型，从再生肝组织中提取总RNA，利用RT-PCR技术扩增出大鼠ALRcDNA，亚克隆于pGEM-T载体，并将DNA序列及其推测的氨基酸序列与Genebank作同源性比较。结果和结论：克隆到大鼠ALRcDNA，经核苷酸序列测定证实序列完全正确，其核苷酸序列没有任何突变，它与酵母ERV1cDNA有较高的同源性，核苷酸序列同源性达54.99％，氨基酸序列同源性达47.01％，并发现氨基酸序列中含有锌指蛋白结合区域。 |
| 关键词: 肝脏再生增强因子 序列分析 |
| DOI： |
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| 基金项目: |
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| Cloning and sequence analysis of the rat augmentor of liver regeneration cDNA |
| 毛积芳,刘鹏飞,缪明永,蔡在龙,陈世敏 |
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| Abstract: |
| Objective: To clone the rat augmentor of liver regeneration (AL R) cDNA and analyse its structure. Methods: The rat ALR cDNA was o btained by using RT-PCR method with total RNA extracted from the regenerating hepatic tissue,then was subcloned into the pGEM-T vector.The sequence of rat A LR cDNA was proved to be correct by sequencing.The rat ALR cDNA and its deduced amino acids sequence were both compared with Genebank database to analyse homol ogy. Results and Conclusion: There isn′t any mutation in cloned r at ALR cDNA.The rat ALR cDNA has higher homology with yeast ERV1 cDNA ,and their homology of DNA and protein is 54.99%and 47.01%respectively. We also find tha t a portion of the rat ALR protein has identity with the binding motif in zinc f inger proteins. |
| Key words: augmentor of liver regeneration seque nce analysis |
