| 摘要: |
| 目的:了解血透患者庚型肝炎病毒(HGV)感染率及与其他肝炎病毒重叠感染的情况。方法:收集57份血透患者血清标本，根据HGV RNA保守片段5′非编码区序列，设计了两对引物，采用逆转录巢式PCR扩增HGV RNA，以及EIA法检测HGV IgG抗体。同时还检测了HBV DNA及HCV IgG抗体。结果:57份标本中，4份HGV RNA阳性(7.0％)，2份HGV IgG抗体阳性(3.5％)。HGV RNA与HGV IgG抗体呈“分离”现象。4份HGV RNA阳性标本中2份为HBV DNA阳性,2份为HCV IgG抗体阳性;其中1份标本HBV DNA和HCV IgG抗体均为阳性。结论:血透患者HGV感染率高于健康献血员和正常人群,HGV可与HBV和HCV重叠感染。 |
| 关键词: 肝炎病毒，庚型 血液透析 抗体，病毒 |
| DOI： |
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| 基金项目: |
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| Detection of serum HGV RNA and HGV IgG antibody in hemodialysis patients |
| 朱诗应,潘卫,崔晓红,戚中田,缪晓辉,许臻 |
| () |
| Abstract: |
| Objective: To determine the HGV infection rate and status of sup er-infection with other hepatitis virus in hemodialysis patients. Metho ds: Fifty seven serum samples were collected from hemodialysis patients. T wo sets of primers were designed and synthesized according to the conserved regi on at 5′ non-coding region of HGV RNA sequence. HGV RNA fragment was amplified by reverse transcript-nested PCR, and IgG antibody to HGV was tested by EIA. M eanwhile, HBV DNA and HCV IgG antibody were tested in all samples. Results : Among 57 serum samples detected, 4 samples were positive for HGV RNA(7.0%) , and 2 were positive for HGV IgG antibody (3.5%).Among samples positive for HGV RNA, 2 were positive for HBV DNA, and 2 were positive for HCV IgG antibody, and one of which was positive for both HBV and HCV markers. Those samples positive for HGV RNA were negative for HGV IgG antibody and vice versa. Concl usion: The infection rate of HGV in hemodialysis patients may be higher th an i n healthy blood donors and healthy population. Patients with HGV infection can b e super-infected with HBV and HCV. |
| Key words: hepatitis G virus hemodialysis antibody, virus |
