| 摘要: |
| 目的：探讨类固醇激素对突触体摄取甘氨酸功能的非基因组作用，以进一步阐明类固醇激.素的生理功能。方法：应用液体闪烁技术检测使用类固醇激素前后小脑或脊髓突触体摄取氚标记甘氨酸的量。结果：皮质酮和孕酮能快速促进小脑突触体摄取甘氨酸，雌二醇和脱氧皮质酮无明显作用，表明类固醇激素作用有特异性。但皮质酮、孕酮、雌二醇和脱氧皮质酮对脊髓突触体均无明显作用。结论：皮质酮和孕酮快速促进小脑突触体摄取甘氨酸是通过非基因组机制起作用的。 |
| 关键词: 甘氨酸 突触体 类固醇激素 非基因组机制 |
| DOI： |
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| 基金项目: |
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| Rapid effects of steroids on glycine uptake in cerebellum and spinal cord synapt osomes |
| 陈宜张,诸秉根,毕钢 |
| () |
| Abstract: |
| Objective: To investigate the nongenomic effects of steroids on the glycine uptake in synaptosomes and to further elaborate on the physiological functio n of steroids. Methods: In synaptosomes of cerebellum or spinal co rd loaded with labeled glycine, the uptakes of glycine without and with steroids were measured by liquid scintillation technique. Results: Cortico sterone and progesterone stimulated the glycine uptake in synaptosomes of cerebe llum, but estradiol and deoxycorticosterone could not, which demonstrated that the effects of steroids were specific. Corticosterone, progesterone, estradiol a nd deoxycorticosterone showed no effects on the glycine uptake in spinal cord sy naptosomes. Conclusion: The mechanisms of the rapid stimulating ef fects of corticosterone and progesterone on the glycine uptake in synaptosomes o f cerebellum are nongenomic. |
| Key words: glycine synaptosomes steroids nongenomic mechanism |
