| 摘要: |
| 目的：研究细胞间粘附分子-1（ICAM-1）反义DNA对内皮细胞ICAM-1表达的抑制作用。方法：构建ICAM-1反义DNA载体，用脂质体转染内皮细胞，TNFα刺激后，流式细胞术检测转染细胞表面ICAM-1蛋白的表达。结果：酶切鉴定证明，1.4kb的ICAM-1DNA片段反向连接到pcDNA3表达载体；流式细胞术检测显示，正常细胞加TNFα刺激后，表面ICAM-1表达明显升高（P<0.01）,转染细胞加TNFα刺激后表面ICAM-1表达无明显升高(P>0.05)，转染细胞表面ICAM-1表达低于正常细胞（P<0.05）。结论：ICAM-1反义DNA体外可抑制细胞ICAM-1表达。 |
| 关键词: 细胞间粘附分子 DNA，反义 载体构建 |
| DOI： |
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| 基金项目: |
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| Inhibitory effect of ICAM-1 antisense DNA on ICAM-1 expression in endothelial cells in vitro |
| 陈蕾,王琪,梅长林,徐洪实 |
| () |
| Abstract: |
| Objective: To observe inhibitory effect of intercellular adhesio n molecule-1(ICAM-1) antisense DNA on ICAM-1 expression in endothelial cells. Methods: ICAM-1 antisense DNA vector was constructed and transfe cted into endothelial cells by lipofectamine reagents. ICAM-1 expression was me asured by flow cytometry in endothelial cells and transfected cells with or with out TNFα. Results: Agarose gel electrophoresis showed 1.4 kb ICAM -1 DNA was inserted into pcDNA3 expression vector in reverse direction. ICAM-1 expression in normal cells with the stimulation of TNFα was significantly high er than without the stimulation(P<0.01). There was no significant increase in ICAM-1 expression in transfected cells with the stimulation of TNFα(P> 0.05). ICAM-1 expression in transfected cells was lower than that in normal cel ls(P<0.05). Conclusion: ICAM-1 expression in endothelial cell s can be inhibited by ICAM-1 antisense DNA in vitro. |
| Key words: intercellular adhesion molecule DNA, antisense vector constru ction |
