摘要: |
目的:研究黄颜木素对激活的永生型大鼠肝储脂细胞--HSC-T6细胞增殖和胶原合成的影响。方法:细胞增殖采用结晶紫染色法检测,胶原合成采用3H-脯氨酸掺入法分析。结果:黄颜木素(6.25-50.00μmol/L)以剂量依赖方式显著抑制血小板源生长因子(PDGF)刺激的HSC-T6细胞增殖,并抑制转化生长因子β1(TGFβ1)诱导的细胞内胶原合成。结论:黄颜木素具有抑制激活的肝储脂细胞增殖和胶原合成的作用。 |
关键词: 黄颜木素 血小板源生长因子 转化生长因子β 肝硬化 |
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Effects of fisetin on proliferation and collagen synthesis of HSC-T6 cells |
钱定华,王杰松,张俊平,张珉 |
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Abstract: |
Objective: To study the effects of fisetin on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line:HSC-T6 cells . Methods: Cell proliferation was measured by crystal violet stain ing assay. Collagen synthesis was determined by 3H-proline incorporation. Results: Fisetin (6.25-50.00 μmol/L)markly inhibited PDGF-stim ulat ed proliferation and TGFβ1-stimulated collagen synthesis of HSC-T6 cells in a concentration-dependent manner. Conclusion: Fisetin could inhi bit actived hepatic lipocyte proliferation and collagen synthesis |
Key words: fisetin platelet-derived growth factor transforming growth f actor beta liver cirrhosis |