| 摘要: |
| 目的：用薄层色谱扫描法检测抗真菌药物氟康唑对真菌麦角固醇生物合成的影响。方法：真菌与不同浓度氟康唑温育24h后计数、称量、皂化，提取真菌中难皂化脂，薄层层析，扫描测定其中的麦角固醇和羊毛固醇含量。结果：随着氟康唑浓度的增加，真菌中的麦角固醇含量逐渐减少，而羊毛固醇含量迅速增加。当抑制85％的麦角固醇生物合成时，真菌生长繁殖的受抑率也在85％左右。结论：薄层色谱扫描法能准确测定真菌中的麦角固醇和羊毛固醇含量，可用于考察抗真菌药物对真菌麦角固醇生物合成的影响，为新化合物的体外抗真菌活性筛选和作用机制研究提供了新方法。 |
| 关键词: 麦角固醇 羊毛固醇 氟康唑 色谱法，薄层 |
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| Effect of fluconazole on ergosterol biosynthesis in fungi by TLC |
| 张万年,孙福红,殷明,姜远英,曹永兵 |
| () |
| Abstract: |
| Objective: To study the effect of fluconazole on ergosterol bios ynthesis in fungi by the method of thin-layer chromatography (TLC). Methods: Saponification and extraction of the nonsaponifiable lipids （NSLs） of fungi were carried out after 24h of incubation with varied concentrations of fluconazole. The components of the NSLs were separated by TLC and the concentr ations of ergosterol and lanosterol were determined. Results: Fluc onazole inhibited ergosterol biosynthesis and caused the concentration of lanost erol increased in a dose-dependent manner in fungi. When fluconazole inhibited ergosterol biosynthesis by 85%, the growth of fungi was also inhibited by about 85%. Conclusion: The effect of antimycotic agents on ergosterol biosynthesis in fungi can be studied by determining the concentrations of ergoster ol and lanosterol by TLC. The method can be used to study the activity and mecha nism of antimycotic agents because of its accuracy and easy operation. |
| Key words: ergosterol lanosterol fluconazole chromatography, thin layer |
