| 摘要: |
| 目的：探讨Wasternblot法对重症肌无力合并胸腺瘤(MGT)患者的血清学诊断价值。方法：分别用蛋白印迹法和酶联免疫法检测33例重症肌无力患者（有瘤16例，无瘤17例）、正常人10例、其他疾病对照18例血清中的人骨骼肌柠檬酸提取物抗体（CAE-Ab）。结果：CAE抗原中3.4万，5.0万，6.0万组分与MGT患者血清结合显色率分别为93.7％（15/16例），75.0％（12/16例），75.0％（12/16例），而其他组无1例结合显色。蛋白印迹法、酶联免疫法对MGT患者检出阳性率分别为16/16，12/16，假阳性率分别为0/17，1/17。结论：蛋白印迹法检测血清中CAE-Ab比酶联免疫法更敏感，特异性高。但蛋白印迹法比酶联免疫法操作复杂，费用较高。 |
| 关键词: 重症肌无力 胸腺肿瘤 印迹法，蛋白质 |
| DOI： |
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| Comparison of Western blotting and ELISA in sera diagnosis of myastheni a gravis with thymoma |
| 张仁琴,涂来慧,王清华 |
| () |
| Abstract: |
| Objective: To improve positive rate of thymoma diagnosis. Methods: Antibodies to CAE (citric acid extract of human skeletal muscle ) were evaluated by Western blotting and ELISA respectively in sera of myastheni a gr avis patients (16 cases with and 17 cases without thymoma),normal adults(10 case s) and other disease patients (18 cases). Results: The rates of CA E-Abs to 3.4×104, 5.0×104, 6.0×104 ingredients of CAE-antigen in MGT patients were 93.7%(15/16),75.0%(12/16), 75.0%(12/16) r espect ively. None of other groups was binding to each of these 3 ingredients. The posi tive rates of MGT patients were 16/16,12/16 respectively. The false positive rat e s were 0/17,1/17 respectively. Conclusion: Western blotting assay was more sensitive and specific than ELISA in evaluating CAE-Abs.But the operation of Western blotting assay is more complicated and expensive than ELISA. |
| Key words: myasthenia gravis thymus, neoplasms blotting, Western |
