| 摘要: |
| 目的:探讨大蒜素诱导卵巢癌细胞株OVCA-3凋亡规律。方法:应用DNA断裂点标记（TUNEL）法、DNA凝胶电泳以及光镜等技术观察有关细胞的凋亡情况。结果:0.01-100μg/ml的大蒜素作用OVCA-3细胞2h及10μg/ml的大蒜素作用OVCA-3细胞不同时间，锥虫蓝染色阳性率均在18.5％以下。TUNEL显示：1-100μg/ml的大蒜素作用OVCA-3细胞2h，即出现典型的细胞凋亡特征；50μg/ml时凋亡率为65.9％。结论:一定剂量范围内的大蒜素可在直接杀伤作用较小的情况下明显诱导OVCA-3细胞凋亡，不会因细胞坏死而引起炎症反应，这对于大蒜素应用于临床将有重要意义。 |
| 关键词: 卵巢肿瘤 大蒜素 细胞凋亡 |
| DOI： |
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| 基金项目: |
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| Allicin induced apoptosis of human ovarian carcinoma cell line OVCA-3 |
| 张登海,黄雪强,潘瑞萍,凌昌全,俞超芹 |
| () |
| Abstract: |
| Objective: To investigate the rule of allicin induced apoptosis.Methods: Terminal deoxylnucleotide transferase directed X-UTP nick and end labelling (TUNEL) assay, DNA gel electrophoresis and light microscopy were used to observe apoptosis. Results: Trypan Blue staining positive rates of OVCA-3 cell were under 18.5%after being exposed to allicin at t he d osage 0.01-100 μg/ml for 2 h and 10 μg/ml for different time. Allicin induced apoptosis of OVCA-3 cells at 1-100 μg/ml for 2 h. Apoptosis peak was shown at 50 μg/ml. Apoptotic cells accounted for 65.9%of the population. Conclusion: Allicin of appropriate dosage induces apoptosis of OVCA-3 cel l, while has little toxicity on OVCA-3 cell. That is important for clinical app lication of allicin. |
| Key words: ovarian neoplasms allicin apoptosis |
