| 摘要: |
| 目的:评价H1受体拮抗剂在哮喘炎症防治中的作用。方法:采用3种治疗方案(生理盐水1 ml/d,酮替芬0.1 mg.kg-1d-1,氯雷他定2 mg.kg-1.d-1),测定3组哮喘豚鼠细胞间粘附分子-1(ICAM-1)、P-选择素(P-S)、白介素8(IL-8)和嗜酸性粒细胞阳离子蛋白(ECP)水平的变化,以及肺功能损害和组织学改变。结果:氯雷他定治疗后,(1)血浆或血清和支气管肺泡灌洗液(BALF)中sICAM-1,sP-S,ECP和BALF中IL-8水平均较对照组下降(P＜0.05);(2)肺组织气道上皮和血管内皮细胞ICAM-1及IL-8表达明显下调,且氯雷他定效果优于酮替芬(P＜0.05);(3)血白细胞ICAM-1表达明显下调(P＜0.05);(4)动态肺顺应性和潮气量显著提高,气道阻力降低(P＜0.05);(5)气道炎症和嗜酸性粒细胞脱颗粒明显被抑制。结论:新一代的H1受体拮抗剂氯雷他定具有多方面抗炎作用,本研究可为临床防治哮喘气道炎症提供实验依据。 |
| 关键词: 哮喘、豚鼠、H1受体拮抗剂、粘附分子 |
| DOI： |
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| Effects of H1-receptor antagonists on the airway inflammation of bronchial asthma |
| 叶曜芩,李寅 |
| () |
| Abstract: |
| Objective:To investigate the effects of H1-receptor (H1R) antagonists on the airway inflammation of asthmatic guinea pigs. Methods:The guinea pigs were divided into 3 groups: loratadine treated group (2 mg.kg-1.d-1),normal saline group (1 ml/d), and ketotifen treated group (0.1 mg.kg-1.d-1). The following tests were performed in 3 groups. (a)The ICAM-1, P-S,IL-8 and ECP values both in plasma (or serum )and BALF were measured by ELISA. (b)The expression of ICAM-1 and IL-8 on the bronchial epithelial cells and pulmonary vascular endothelial cells were measured by immunohistochemistry staining. (c)The expression of ICAM-1 on the leukocytes were measured by FCM. (d)The lung functions(Vt, Cdyn, Raw)were tested. (e)The airway tissues were examined by microscope and TEM. Results:(a)Apart from the values of IL-8 in BALF level of ketotifen group, the values of sICAM-1, sP-S,IL-8 and ECP were reduced by 2 kinds of H1R antagonists(loratadine and ketotifen)both in the plasma(or serum)and BALF of asthmatic animals. (b)The expressions of ICAM-1 and IL-8 on the brochial epithelial cells and pulmonary vascular endothelial cells in asthmatic animals were down-regulated by 2 kinds of H1R antagonists especially loratadine. (c)The expressions of ICAM-1 on the leukocytes were down-regulated by 2 kinds of H1R antagonists. (d)The lung functions (Vt, Cdyn, Raw) of the asthmatic animals were improved by 2 kinds of H1R antagonists.(e)The infiltration in the bronchial mucosa and the degranulation of eosinophils were reduced by 2 kinds of H1R antagonists. |
| Key words: asthma guinea pig adhesion molecule H1-receptor antagonist |
