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小鼠脾脏褪黑素受体及其昼夜节律的研究
彭树勋,邵福源,赵瑛
0
()
摘要:
目的:研究小鼠脾脏是否存在褪黑素(M)结合位点,该结合位点是否存在昼夜节律。方法:应用放射配体结合法检测小鼠脾脏M结合位点(MR)。每4 h处死一批动物,研究不同时间脾脏MR的差异;分别持续光照及注射M 21 d,观察MR结合量及昼夜节律的改变。结果:小鼠脾脏存在MR,其特点为低结合容量,高亲和力,对M具高度特异性,符合特异结合位点的基本条件;昼夜节律研究显示,MR结合量峰值在12:00-16:00,谷值在0:00-4:00,F检验峰谷值间差异显著(P<0.01)。结论:小鼠脾脏存在MR,其MR有昼夜节律变化;持续光照及注射M不影响MR节律,表明MR昼夜节律的机制是自主的,具生理学意义。
关键词:  褪黑素、结合位点  脾脏  昼夜节律
DOI:
基金项目:
Circadian rhythm of melatonin receptor in the mouse spleen
彭树勋,邵福源,赵瑛
()
Abstract:
Objective: To determine whether there exists melatonin receptor (MR) in the mouse spleen and the circadian rhythm (CR) in MR. Methods: Specific binding of melatonin to the mouse spleen was measured by radioligand binding assay. Results: The results revealed the maximum binding capacity (Bmax) was (1.25±0.21) fmol/mg protein and equilibrium constant (Kd)was (59.5±10.1) pmol/L. The peak values of Bmax of MR was from 12:00 to16:00 and the trough values was between 0:00 to 4:00. The difference between them was significant statistically. CR of MR showed no change when continued light (3 weeks) and injected melatonin in peritoneum (3 weeks). Conclusion: MR is present in the mouse spleen and there exists CR of MR in the mouse spleen. It is indicated that CR of MR in spleen is independent from that of melatonin.
Key words:  melatonin binding site  spleen  circadian rhythm