| 摘要: |
| 目的：通过遗传学育种使外源基因整合位点随机的HBV转基因小鼠成为单一整合位点的纯合子转基因小鼠。方法：产生founder小鼠以后，将正常小鼠与转基因小鼠交配，通过PCR，Dot-blotting，Southern-blotting等方法检测 HBV DNA在转基因小鼠体内的整合状况，阳性率达50％左右后，进行近亲交配。结果：共得到了7代HBV转基因小鼠，其中包含有整合HBV基因纯合子的转基因小鼠。结论：外源基因在转基因小鼠体内可以稳定遗传，并得到了整合有HBV基因的纯合子转基因小鼠。 目的：通过遗传学育种使外源基因整合位点随机的HBV转基因小鼠成为单一整合位点的纯合子转基因小鼠。方法：产生founder小鼠以后，将正常小鼠与转基因小鼠交配，通过PCR，Dot-blotting，Southern-blotting等方法检测 HBV DNA在转基因小鼠体内的整合状况，阳性率达50％左右后，进行近亲交配。结果：共得到了7代HBV转基因小鼠，其中包含有整合HBV基因纯合子的转基因小鼠。结论：外源基因在转基因小鼠体内可以稳定遗传，并得到了整合有HBV基因的纯合子转基因小鼠。 目的：通过遗传学育种使外源基因整合位点随机的HBV转基因小鼠成为单一整合位点的纯合子转基因小鼠。方法：产生founder小鼠以后，将正常小鼠与转基因小鼠交配，通过PCR，Dot-blotting，Southern-blotting等方法检测 HBV DNA在转基因小鼠体内的整合状况，阳性率达50％左右后，进行近亲交配。结果：共得到了7代HBV转基因小鼠，其中包含有整合HBV基因纯合子的转基因小鼠。结论：外源基因在转基因小鼠体内可以稳定遗传，并得到了整合有HBV基因的纯合子转基因小鼠。 |
| 关键词: 肝炎病毒，乙型 小鼠，转基因 纯合子 |
| DOI： |
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| 基金项目: |
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| The generation of hepatitis B virus transgenic mice homozygote |
| 胡以平,郝光荣,孙伟,王新民,李建秀,戴德顺,胡卫江 |
| () |
| Abstract: |
| Objective： To obtain the homozygote of HBV transgenic mice with unique integrated site by genetic breeding. Methods: The positive transgenic mice were mated with the normal mice of the same line. And the target gene integration ratio were tested by PCR, Dot-blotting and Southern-blotting, and when the integration ratio was almost 50%, the positive mice were mated with inbreeding. Results: The 7 generations of HBV transgenic mice were obtained, and the homozygote of HBV transgenic mouse was obtained. Conclusion: The HBV DNA can be transmitted in the transgenic mice stably, and the homozygote of HBV transgenic mouse was obtained. |
| Key words: hepatitis B virus mice, transgenic homozygote |
