| 摘要: |
| 目的：为使外源性TNF,IFN基因和内源性主要组织相容性复合物Ⅰ类(MHCⅠ)分子基因表达呈现“正反馈”式放大效应，构建在 HLA-B7启动子调控、IRES连接下协同表达TNFα和IFNβ基因的重组腺病毒载体。方法和结果:从pGL3B7TNF及pIRIF中切下B7pro-TNF和IRES-IFNβ基因片段，先后插入pBluescript Sk (+), 构建成pBLB7TNIRIF。回收TNFα-IRES-IFNβ基因片段，连入AdCMVLink1中，构建成CMV启动子驱动表达的AdCMVTNIRIF(+)。回收B7pro-TNF-IRES-IFNβ基因片段,连入缺失E1和E3区及启动子的AdBglⅡ中, 构建成HLA-B7启动子驱动表达的AdB7TNIRIF(-)。结论：此两种载体为“正反馈”式放大肿瘤免疫原性提供一条新途径。 |
| 关键词: 干扰素、肿瘤坏死因子、HLA-B7抗原启动子、腺病毒载体 |
| DOI： |
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| 基金项目: |
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| Construction of adenoviral vector increasing tumor immunogenicity by enhancing gene expression of TNF,IFN,MHC Ⅰmolecule |
| 杜平,陈秋莉,段芳龄,曹广文,杨淑英 |
| () |
| Abstract: |
| Objective:：To increase tumor immunogenicity by enhancing gene expression of TNF,IFN,MHC Ⅰ molecule , adenoviral vectors containing HuTNFα and IFNβ cDNA driven by HLA-B7 inducible promoter and CMV promoter were constructed, respectively. Methods and Results：The 2 fragments HLA-B7 promoter-TNF and IRES-IFN were isolated from and pGL3B7TNF and pIRIF and insert to pBluescript Ⅱ Sk (+) to generate pBLB7TNIRIF.TNFα-IRES-IFNβ was isolated and ligated to AdCMVLink1 to generate AdCMVTNIRIF(+), in which gene transcription was driven by CMV promoter.B7pro-TNFα-IRES-IFNβ was isolated and ligated to AdBglⅡto generate AdB7TNIRIF(-),in which gene expression was driven by HLA-B7 promoter. Conclusion：The constructed vectors can be a new route for enlarging tumor immunogenicity. |
| Key words: interferon tumor necrosis factor HLA-B7 promoter adenoviral vector |
