| 摘要: |
| 目的:观察咬合力改变对大鼠牙周膜成纤维细胞凋亡及其增殖细胞核抗原(PCNA)表达的影响,探讨牙周膜改建的可能机制.方法:拔除健康雄性SD大鼠右上颌第一、二、三磨牙,建立左下颌磨牙咬合力改变动物模型,分别于拔牙后6、12 h及1、2、3、5、7、14、28 d处死大鼠(n=6),取牙槽骨组织,进行H-E染色观察;TUNEL法观测牙周膜成纤维细胞的凋亡情况,免疫组织化学染色检测其PCNA表达.另设正常咬合力大鼠作为对照组(n=6).TUNEL法及免疫组织化学染色结果采用二级计分法,根据染色强度及阳性细胞的数目进行计分取平均值.结果:成功建立咬合力改变雄性SD大鼠模型.H-E染色结果显示与拔牙大鼠相比,正常对照组大鼠牙周膜结构较致密,纤维排列有序,牙槽骨骨壁较平.TUNEL法观察显示拔牙后12 h牙周膜成纤维细胞凋亡达峰值(252.67±6.62),明显高于正常对照组(7.17±2.32,P<0.01),其后开始下降,至28 d基本恢复正常(8.50±1.87).免疫组化染色表明PCNA的表达也呈类似趋势,拔牙后3 d表达最高(246.00±7.21),此后开始下降至正常(5.67±2.16,P<0.01).结论:咬合力改变可能通过加速牙周膜成纤维细胞的凋亡,促进细胞增殖,从而共同完成牙周膜的改建. |
| 关键词: 咬合力、牙周膜、成纤维细胞、细胞凋亡、增殖细胞核抗原 |
| DOI：10.3724/SP.J.1008.2006.00484 |
|
| 基金项目: |
|
| Influence of occlusal force change on apoptosis of periodontal ligament fibroblast and expression of proliferating nuclear antigen |
| 王国栋,姜晓钟,何玉林,WANG Guo-dong,JIANG Xiao-zhong,HE Yu-lin |
| () |
| Abstract: |
| Objective： To study the influence of occlusal force change on apoptosis of periodontal ligament fibroblast （PDLF） and expression of proliferating nuclear antigen （PCNA） in the fibroblasts, so as to explore the possible mechanism for remodeling of periodontal ligament. Methods： Animal model of occlusal force change was established by extracting the right first, second and third maxillary molars in male SD rats. The rats were sacrificed at 6, 12 hours and day 1, 2, 3, 5, 7, 14, and 28 d after extracting the teeth（n = 6）, and their alveolar bone tissues were harvested. H-E staining was used to observe the morphologic changes of the alveolar bone tissues. TUNEL method and immunohistochemistry were used to detect apoptosis and PCNA expression in PDLF. The rats with normal occlusal forces were used as control（n= 6）. The results were assigned a mean score based on the percentage and the intensity of cells positively stained for TUNEL and PCNA. Results：Animal model of occlusal force change was successfully established, H-E staining showed that the control group had more pycnotic periodontal ligament, orderly fibres, and flatter alveolar bone than the model group. TUNEL result showed that the cell apoptosis reached its peak at 12 haffer extracting the teeth （252.67 ± 6.62）, significantly higher than that of control group （7.17 ± 2.32, P〈0.01 ）, then gradually decreased to normal level on day 28 after extraction （8. 50 ± 1.87）. Immunohistochemical staining showed that the expression of PCNA had a similar tendency of apoptosis：PCNA expression peaked on day 3 after extraction （246.00 ± 7.21） and then gradually decreased to normal level （5.67 ± 2.16, P〈0.01）. Conclusion： Changes of occlusal force can facilitate the PDLF apoptosis and promote PDLF proliferation, contributing to the remodeling of periodontal ligament |
| Key words: bite force periodontal ligament fibroblasts apoptosis proliferating cell nuclear antigen |
