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  • 韩庆林1,苟三怀1*,王良哲2,康健1,王琪1.聚乙烯亚胺介导BMP-7基因体内转染促进老年大鼠骨折愈合[J].第二军医大学学报,2008,29(5):0514-0518    [点击复制]
  • HAN Qing-lin1,GOU San-huai1*,WANG Liang-zhe2,KANG Jian1,WANG Qi1.Polyethylenimine-mediated BMP-7 gene transfection promotes fracture healing in elderly rats[J].Acad J Sec Mil Med Univ,2008,29(5):0514-0518   [点击复制]
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聚乙烯亚胺介导BMP-7基因体内转染促进老年大鼠骨折愈合
韩庆林1,苟三怀1*,王良哲2,康健1,王琪1
0
(1.第二军医大学长征医院骨科,上海 200003;2.第二军医大学长征医院病理科,上海 200003)
摘要:
目的:观察聚乙烯亚胺(polyethylenimine,PEI)作为基因载体介导BMP-7基因体内转染促进骨折愈合的效果。方法:20月龄雄性SD大鼠随机分为3组:BMP-7+PEI治疗组、生理盐水对照组及BMP-7对照组(n=20)。所有大鼠建立右股骨干骨折模型,BMP-7+PEI治疗组于骨折断端经皮注射pcDNA3.1-BMP-7/PEI混合试剂,生理盐水及BMP-7对照组分别注射等量生理盐水及pcDNA3.1-BMP-7。治疗后2、4、8周观察X线片、H-E染色、Ⅰ型胶原免疫组化染色、生物力学和骨密度检测结果,判断骨折愈合情况。结果:X线及H-E染色结果表明,3组老年大鼠骨折后第8周均未获得愈合,但BMP-7+PEI治疗组骨痂生长情况优于生理盐水及BMP-7对照组,断端间出现部分骨性连接。Ⅰ型胶原染色表明,与生理盐水及BMP-7对照组相比,BMP-7+PEI治疗组Ⅰ型胶原染色深、分布范围较大。抗弯曲强度和骨密度检测结果表明,BMP-7+PEI治疗组各项指标均优于对照组,差异有统计学意义(P<0.05或0.01)。结论:PEI介导BMP-7基因体内转染可有效促进老年大鼠骨折愈合。
关键词:  聚乙烯亚胺;BMP-7;转染;骨折  老年大鼠
DOI:10.3724/SP.J.1008.2008.00514
投稿时间:2007-06-11
基金项目:
Polyethylenimine-mediated BMP-7 gene transfection promotes fracture healing in elderly rats
HAN Qing-lin1,GOU San-huai1*,WANG Liang-zhe2,KANG Jian1,WANG Qi1
(1. Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;2. Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200003)
Abstract:
Objective:To observe polyethylenimine-mediated BMP-7 gene transfection in promoting fracture healing in elderly rats. Methods:Male SD rats (20-month-old) were randomly divided into 3 groups: group A received BMP-7 gene therapy mediated by polyehtylenimine, group B was treated with normal saline and group C was treated with BMP-7 plasmid without polyethylenimine. Right femoral shaft fracture model was established in all rats. Animals in group A received transdermal injection of pcDNA3.1-BMP-7/PEI; those in group B and C received the same volume of normal saline and pcDNA3.1-BMP-7, respectively. X-ray photography, histological observation (H-E staining), immunohistochemical staining of collagen type Ⅰ, biomechanical and bone mineral density test were employed to assess the healing of fracture 2, 4, and 8 weeks after treatment. Results: The results of X ray and H-E staining showed no fracture healing in the 3 groups during the 8th week after fracture; however, the growth of coloboma in group A was better than that of the other 2 groups, with partial bone union between the fracture ends. Staining of collagen type Ⅰ showed deeper, wider staining in group A compared with the other 2 group. Anti-bending intensity and bone mineral density tests showed that the parameters in group A were better than those of the other 2 groups (P<0.05 or 0.01). Conclusion: Polyethylenimine-mediated hBMP-7 gene transfer can effectively promote fracture healing in elderly rats.
Key words:  polyethylenimine  BMP-7  transfection  fracture  old rats