| 摘要: |
| 目的 通过cell counting kit-8(CCK-8)检测不同粒径硒化镉(CdSe/ZnS)量子点对人恶性黑素瘤细胞A375、A375.s2以及正常人表皮细胞HaCaT的细胞毒性,研究硒化镉量子点对黑素瘤细胞生长的影响。方法 分别取处于对数生长期的A375、A375.s2以及HaCaT细胞铺入96孔板内,次日分别向铺有细胞的孔内加入浓度为162、81、54、40.5、27、10.125、4.05、2.025、1.012 5 nmol/L水溶性量子点(QDs)-605与QDs-545进行孵育,24 h后每孔加入10 μl CCK-8,测定2种硒化镉量子点对A375、A375.s2及HaCaT细胞增殖的影响。结果 CCK-8法显示,随着QDs-605与QDs-545浓度的增加,A375和A375.s2细胞存活率随之下降。不同浓度的QDs-605与QDs-545作用于A375、A375.s2细胞,24 h后其细胞存活率均低于对照组(P<0.05,P<0.01),但是对HaCaT细胞没有抑制作用。结论 硒化镉量子点对A375、A375.s2人黑素瘤细胞有一定的抑制作用,而对HaCaT正常人表皮细胞抑制作用不明显。 |
| 关键词: 硒化镉量子点； 黑素瘤； 表皮细胞； 细胞增殖 细胞毒性 |
| DOI：10.3724/SP.J.1008.2010.0465 |
| 投稿时间：2010-01-27修订日期：2010-04-21 |
| 基金项目:上海市科委纳米技术专项基金(0852nm04200). |
|
| Cytotoxic effect of CdSe/ZnS quantum dots on melanoma cells and normal human epidermal cells |
| SONG Fang-ming, ZHENG Hong, LOU Zi-yang* |
| (Center of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| Objective To study the cytotoxic effect of the CdSe/ZnS quantum dots of different diameters on human malignant melanoma cell line A375, A375.s2 and normal human epidermal cell line HaCaT using cell counting kit-8（CCK-8）, so as to study the influence of CdSe/ZnS quantum dots on growth of melanoma cells. Methods Log phase A375,A375.s2 and HaCaT cells were plated into 96-well plates. After a 24 h incubation, the prepared QDs-605 and QDs-545 solutions of different concentrations (162, 81, 54, 40.5, 27, 10.125, 4.05, 2.025, and 1.012 5 nmol/L) were added into the wells. After another 24 h incubation, CCK-8 was added into the well (10 μl/well).Then the cytotoxicities of CdSe/ZnS quantum dots on the proliferation of A375, A375.s2 and HaCaT cells were determined by MTT assay. Results We found that with the increase of QDs-605 and QDs-545 concentrations, the survival rates of A375, A375.s2 cells were decreased. A day after A375, A375.s2 cells were treated with different concentrations of QDs-605 and QDs-545, the cell survival rate was significantly lower than that of the control group(P<0.05,P<0.01); however, different concentrations of QDs-605 and QDs-545 did not inhibit the proliferation of HaCaT cells.Conclusion CdSe/Zns quantum dots have inhibitory effect on the growth of human malignant melanoma cell line A375, A375.s2, and have no noticeable effect on normal human epidermal cell line HaCaT. |
| Key words: Supported by the Nano-technology Foundation of Shanghai Science and Technology Committee (0852nm04200). |
