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| 低能量激光调控大鼠正畸保持期骨改建因子的表达 |
| 冉宇婷1,于文雯2,董晓曦3,韩开放4,常笑4,张锡忠2，4* |
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| (天津市口腔医院儿童口腔科, 天津 300041;天津市口腔医院正畸科, 天津 300041;中国医学科学院生物医学工程研究所激光医学实验室, 天津 300192;南开大学医学院, 天津 300071;天津市口腔医院正畸科, 天津 300041;1. 天津市口腔医院儿童口腔科, 天津 300041) |
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| 摘要: |
| 目的 探究低能量激光对大鼠牙齿移动后保持期骨改建因子的影响。方法 80只Wistar大鼠随机分为空白对照组和4个模型组（复发组、保持组、保持＋激光组、复发＋激光组），每组16只。空白对照组不进行主动加力，不施加任何措施。其余4组制作第一磨牙近中移动模型，在21 d主动加力结束后拆除口内装置；然后，复发组不安装保持装置，保持组安装保持装置，保持＋激光组安装保持装置并隔日照射低能量激光，复发＋激光组不施加保持装置但隔日照射低能量激光。在主动加力21 d后进入保持期的第2、5、10、15天处死大鼠，提取大鼠第一磨牙周围2 mm组织的总RNA，采用qPCR法检测Runt相关转录因子2（Runx2）、碱性磷酸酶（ALP）、Ⅰ型胶原蛋白（COLⅠ）、活化T细胞核因子（NFATc）、组织蛋白酶 K（CTSK）、基质金属蛋白酶 9（MMP9）等6个骨改建因子的mRNA表达。结果 4个模型组大鼠的6个骨改建因子的mRNA表达均较空白对照组呈升高趋势，在大部分时间点差异有统计学意义（P均＜0.05）。复发组的6个骨改建因子的mRNA表达较保持组呈升高趋势，在各时间点差异均有统计学意义（P均＜0.01）。随着时间延长，保持＋激光组6个骨改建因子的mRNA表达较保持组升高，从第5天开始差异均有统计学意义（P均＜0.05），第10天差异最为明显。复发＋激光组除MMP9外的5个骨改建因子的mRNA表达在大部分时间点均较复发组升高（P均＜0.05）。结论 在大鼠第一磨牙的固定保持过程中，低能量激光的干预增加了骨组织改建因子的表达，加速了牙槽骨的改建。 |
| 关键词: 正畸 保持 骨改建因子 低能量激光 复发 |
| DOI：10.16781/j.CN31-2187/R.20210474 |
| 投稿时间：2021-05-06修订日期：2022-01-06 |
| 基金项目:天津市卫生健康委员会科技项目(ZC0001),天津市重点培育专科(津卫科教[2018]268号). |
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| Low-level laser regulates the expression of bone remodeling factors during orthodontic retention in rats |
| RAN Yu-ting1,YU Wen-wen2,DONG Xiao-xi3,HAN Kai-fang4,CHANG Xiao4,ZHANG Xi-zhong2，4* |
| (Department of Pediatric Stomatology, Tianjin Stomatological Hospital, Tianjin 300041, China;Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin 300041, China;Laser Medical Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China;School of Medicine, Nankai University, Tianjin 300071, China;Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin 300041, China;1. Department of Pediatric Stomatology, Tianjin Stomatological Hospital, Tianjin 300041, China) |
| Abstract: |
| Objective To investigate the effect of low-level laser on periodontal bone remodeling factors during orthodontic retention after tooth movement in rats.Methods A total of 80 Wistar rats were randomly divided into blank control group and 4 model groups (relapse group,retention group,retention+laser group,and relapse+laser group),with 16 rats in each group.In the blank control group,no active force was applied and no measures were taken.The first molar mesial movement model was constructed in the other 4 groups,and the intraoral device was removed after 21 d of active force application.The relapse group was equipped without retention device,the retention group was equipped with retention device,the retention+laser group was equipped with retention device and irradiated with low-level laser every other day,and the relapse+laser group was equipped without retention device but irradiated with low-level laser every other day.The rats were sacrificed on the 2nd,5th,10th and 15th day of the retention period after 21 d of active force application,and the total RNA of 2-mm tissues around the first molar was extracted.The mRNA expression of bone remodeling factors (Runt-related transcription factor 2[Runx2],alkaline phosphatase[ALP],type Ⅰ collagen[COLⅠ],nuclear factor of activated T cell[NFATc],cathepsin K[CTSK],and matrix metalloproteinase 9[MMP9]) were detected by quantitative polymerase chain reaction (qPCR).Results The mRNA expression of the 6 bone remodeling factors in the 4 model groups was higher than that in the blank control group,and the differences were statistically significant at most time points (all P<0.05);the mRNA expression of the 6 bone remodeling factors in the relapse group was significantly higher than that in the retention group at each time point (all P<0.01).As time went on,the mRNA expression of 6 bone remodeling factors in the retention+laser group was higher than that in the retention group,and the difference was significant from the 5th day (all P<0.05) and peaked on the 10th day.Except for the MMP9,the mRNA expression of the other 5 bone remodeling factors in the relapse+laser group was higher than that in the relapse group at most time points (all P<0.05).Conclusion In the retention process of the first molar in rats,the low-level laser can increase the expression of bone remodeling factors and accelerate the remodeling of alveolar bone. |
| Key words: orthodontics retention bone remodeling factors low-level laser recurrence |
