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  • 方之扬,葛绳德,陈玉林,戴军.烫伤大鼠中枢氨基酸递质和心血管功能改变的关系[J].第二军医大学学报,1999,20(4):    [点击复制]
  • 方之扬,葛绳德,陈玉林,戴军.Relation between central amino acid transmitter and changes of cardiovascular function in burned rats[J].Acad J Sec Mil Med Univ,1999,20(4):   [点击复制]
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烫伤大鼠中枢氨基酸递质和心血管功能改变的关系
方之扬,葛绳德,陈玉林,戴军
0
()
摘要:
目的:观察烫伤早期大鼠下丘脑氨基酸递质含量及γ-氨基丁酸(GABA)受体的变化,及其对心血管指标的影响。方法:制备大鼠30%总体表面积Ⅲ度烫伤模型,采用脑组织游离氨基酸自动分析法测定烫伤大鼠下丘脑氨基酸递质相对含量,运用神经元突触膜放射配体结合法测定GABA受体功能,侧脑室内注射印防己毒素和海人酸,运用四导生理仪记录血压和心肌力学指标。结果:烫伤早期大鼠下丘脑GABA含量明显增加,谷氨酸减少,其他氨基酸递质含量无明显变化;GABA受体最大结合容量明显增加,平衡解离常数无明显改变。烫伤大鼠中枢GABA作用的阻断可明显回升其血压、左心室收缩压、左心室内压最大变化速率,谷氨酸的激活也有一定的作用。结论:大鼠烫伤早期中枢GABA及谷氨酸递质改变可能是其对心血管功能减退的影响因素之一。
关键词:  γ-氨基丁酸  下丘脑  心血管功能  烧伤
DOI:
基金项目:
Relation between central amino acid transmitter and changes of cardiovascular function in burned rats
方之扬,葛绳德,陈玉林,戴军
()
Abstract:
Objective: To observe the change of amino acid transmitter and gamma-aminobutyric acid (GABA) receptor in the hypothalamus of burned rats during early stage, and to investigate its effect on the cardiovascular parameter of burned rats. Methods: 30%TBSA Ⅲ degree burn model of rat was made. Concentration of amino acid transmitter in the hypothalamus of burned rats was determined by auto-analysis of free amino acid. Radioligand binding assay was adopted to determine GABA receptor. The drug was injected into lateral ventricle (LV). Cardiovascular parameter was measured with four leads physiological recording instrument. Results: Concentration of GABA was significantly elevated, concentration of glutamate was reduced and there was no change of other amino acid transmitter in the hypothalamus of burned rats during early stage. Although maximum binding of GABA receptor increased significantly, there was no change of dissociation costant. Picrotoxin injection into LV may be significantly elevate MAP,LVSP,±dp/dtmax of burned rats. There were also limited effects of kinic acid (KA) on these parameters. Conclusion: The change of central GABA and glutamate transmitter may be a effecting factor on cardiovascular function failure of burned rats during early stage
Key words:  gamma-aminobutyric acid  hypothalamus  cardiovascular function  burns