| 摘要: |
| 目的:观察葛根素对培养人主动脉内皮细胞脂质过氧化的保护作用。方法：建立人主动脉内皮细胞体外培养模型，在培养液中加入0.1×10-4和1.0×10-4mol/L联胺，致内皮细胞脂质过氧化损害，即刻或30min后加入葛根素0.3mg/孔，测定噻唑蓝摄取率、乳酸脱氢酶(LDH)、丙二醛(MDA)和凝血?蓖？TXA2)含量。结果：两种浓度的联胺使LDH，MDA和TXA2含量增加，使噻唑蓝摄取率下降，加入葛根素后可使上述结果逆转。结论：葛根素对脂质过氧化致内皮细胞的损害具保护作用。 |
| 关键词: 内皮细胞、联胺、葛根素 脂质过氧化 |
| DOI： |
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| Protective effect of puerarin on cultured human aortic endothelial cells injury caused by lipid peroxide |
| 秦永文,沈茜,章同华,丁继军 |
| () |
| Abstract: |
| Objective: To observe the protective effect of puerarin on cultured human aortic endothelial cells (ECs) injury caused by lipid peroxide. Methods:ECs were treated with diamide to stimulate and faciliate lipid peroxidation. The diamide concentration was 0.1×10-4 and 1.0×10－4mol/L. Then 0.3mg puerarin was added immediately or 0.5h later. Results: Puerarin decreased the LPO and TXA2, then the MTT increased and LDH release decreased. Conclusion: Puerarin reduces the injury of ECs caused by lipid peroxide.It might play an important role in antiatherongenesis. |
| Key words: endothelial cell diamide puerarin lipid peroxidation |
