| 摘要: |
| 目的：研究γ射线照射后小鼠骨髓造血细胞凋亡及其与Fas 表达的关系。方法：采用电镜、DNA琼脂糖凝胶电泳及流式细胞术观察小鼠经60Co γ射线照射后不同时间的骨髓造血细胞凋亡及Fas的表达情况。结果：在照射后4 h细胞即出现核固缩、染色质凝集；DNA琼脂糖凝胶电泳有大量的小分子片段，呈典型“梯状”图谱；流式细胞术分析表明,凋亡细胞百分率在照射后4 h明显增加，8 h达到高峰；照后4 h，Fas表达显著增强。结论：γ射线照射后可引起骨髓造血细胞凋亡，Fas可能参与了辐射诱导的造血细胞凋亡过程。 |
| 关键词: 造血细胞 细胞凋亡 Fas表达 γ射线 |
| DOI： |
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| 基金项目: |
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| Apoptosis of bone marrow hematopoietic cell and Fas expression in 60Coγ irradiated mice |
| 张玲珍,李雨,闵锐,蔡建明,吴玮,项莺松,杨如俊,李百龙 |
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| Abstract: |
| Objective:To study the apoptosis of bone marrow hematopoietic cells and Fas expression in 60Coγ irradiated mice. Methods: Mice were whole-body-irradiated with 4.0 Gy γ-rays.The bone marrow hematopoietic cells were analyzed by electron microscopy(EM),electrophoretic analysis of DNA and flow cytometry. Results:EM showed that there were nuclear contraction and chromatin condensation.Agarose gel electrophoresis of DNA showed ladder pattern.The peak percentage of apoptotic cells occurred 8 h after irradiation. Fas expression significantly increased in bone marrow hematopoietic cells than that of control group 4 h after irradiation. Conclusion: The results indicate that apoptosis occurs in bone marrow hematopoietic cells of mouse with γ-irradiation.The expression of Fas may play a role in raidation-induced apoptosis of hematopoietic cells. |
| Key words: hematopoietic cell apoptosis Fas expression gamma rays |
