| 摘要: |
| 目的：测定青葙子中青葙苷A和青葙苷B的含量。 方法：HPLCELSD法，色谱柱(4.6 mm×250 mm，5 μm)，流动相为乙腈0.1%冰乙酸水溶液梯度洗脱，以蒸发光散射检测器(ELSD)检测。 结果：青葙苷A在2.5～30.0 μg范围内与峰面积呈良好的线性关系，回收率为99.89%，RSD为1.85% (n=6)；青葙苷B在1.25～15.0 μg范围内与峰面积呈良好的线性关系，回收率为98.98%，RSD为1.78% (n=6)。8个市售青葙子样品中青葙苷A和青葙苷B的含量存在显著差异，分别在(0.051 2±0.001 6)%～(0.114 3±0.000 8)%和(0.010 9±0.001 9)%～(0.081 5±0.000 9)%范围内。 结论：本研究首次建立了用HPLCELSD法测定青葙子有效成分的方法，该方法定量准确、操作简便。 |
| 关键词: 青葙子 青葙苷A 青葙苷B 色谱法，高压液相 蒸发光散射检测器 |
| DOI：10.3724/SP.J.1008.2007.01245 |
| 投稿时间：2007-04-30 |
| 基金项目:上海市中药现代化专项基金（06DE19715）；军队“十一五”中医药研发推广专项基金（2006172003）. |
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| HPLC-ELSD in determination of celosin A and celosin B contents in Semen celosiae |
| WANG Ying1,2,GUO Mei-li1*, WANG Xiao-kang2, YIN Jun2 |
| (1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2. Department of Pharmacognosy, College of Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016) |
| Abstract: |
| Objective：To determine the contents of celosin A and celosin B in Semen celosiae.Methods: HPLC was carried out with an Agilent Alliance, Model 1100, equipped with Elite ODS C18 column (4.6 mm×250 mm，5 μm) and evaporated light scattering detector (ELSD). The mobile phase (acetonitrile0.1% glacial acetic acid) was eluted in gradient mode.Results: Celosin A and the peak area showed a good linearship within the range of 2.530.0 μg, with the recovery rate being 99.89% (n=6) and RSD being 1.85%. Celonsin B and the peak area showed a good linearship within the range of 1.2515.0 μg, with the recovery rate being 98.98% (n=6) and the RSD being 1.78%. Contents of celosin A and celosin B in 8 Semen celosiae samples on medical markets were obviously different from each other, ranging from(0.051 2±0.001 6)% to (0.114 3±0.000 8)% and (0.010 9±0.001 9)% to (0.081 5±0.000 9)%.Conclusion: We have established an HPLCELSD method for determining the effective contents of Semen celosiae; the method is accurate and simple. |
| Key words: Semen celosiae celosin A celosin B chromatography,high pressure liquid evaporative light scattering detector |
