| 摘要: |
| 目的：观察异质性胞核核糖核蛋白K(heterogeneous nuclear ribonucleoprotein K,hnRNP K)在肝细胞癌（hepatocellular carcinoma,HCC)组织中的表达及在不同密度肝癌细胞(PLC/PRF/5)中的亚细胞定位。方法：免疫组化染色观察肝细胞癌组织中hnRNP K的表达；Western印迹检测肝癌组织（n=30）及对应癌旁组织hnRNP K蛋白表达差异；应用细胞免疫组化和Western印迹法比较不同密度肝癌细胞hnRNP K蛋白的亚细胞定位及表达。结果：肝细胞癌组织hnRNP K蛋白主要表达于细胞核。hnRNP K蛋白表达在30例的肝癌组织中有21例高于其对应的癌旁组织；20例HBV阳性肝癌组织有16例高于其对应的癌旁组织,而10例HBV阴性肝癌组织仅有5例表达高于癌旁组织。细胞免疫组化结果表明,hnRNP K主要表达于PLC细胞核中,并且随密度（汇合率为30%、50%、90%）的上升,其表达量逐渐上升。Western印迹结果表明,不同密度（汇合率为30%、50%、70%、90%）肝癌细胞的细胞质中,hnRNP K的表达量无统计学差异；而在细胞核中,随着细胞密度的增大,hnRNP K的表达量逐渐升高。结论：肝癌组织细胞核hnRNP K表达上调,且随着肝癌细胞密度的增加,其表达会逐渐增强,HBV感染可能会促进其表达。 |
| 关键词: 肝细胞癌 异质性胞核核糖核蛋白K 乙型肝炎病毒 |
| DOI：10.3724/SP.J.1008.2008.00020 |
| 投稿时间：2007-07-20修订日期：2008-01-17 |
| 基金项目:上海市自然科学基金（04ZR14108）,国家自然科学基金（30400244）. |
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| Expression of heterogeneous nuclear ribonucleoprotein K in hepatocellular carcinoma tissues and cell line of different cell densities |
| FAN Wei-wei, YU Yan-ting,HONG Yi,TAN Ye-xiong, WANG Hong-yang* |
| (International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, China) |
| Abstract: |
| Objective：To investigate the expression changes and the subcellular localization of heterogeneous nuclear ribonucleoprotein K (hnRNP K) in hepatocellular carcinoma (HCC) and cell line of different cell densities. Methods: The expression of hnRNP K in HCC was detected by immunohistochemical staining. The differential expression of hnRNP K in the HCC tissue (n=30) and paired paracancerous tissue was identified by Western blotting assay. The expression and sub-cellular location of hnRNP K in HCC cell line of different cell densities were determined by immunocytochemical staining and Western blotting assay. Results: Expression of hnRNP K was mainly detected in the nucleus of HCC cells. Twenty-one of the 30 hepatic cancer tissues had higher hnRNP K expression than the paired paracancerous tissues. Sixteen of the 20 HCC samples with HBV infection had an increased expression of hnRNP K; in the 10 HCC samples without HBV infection,5 had an increased expression of hnRNP K. Immunocytochemical staining and Western blotting assay both showed that the expression of hnRNP K was increased in the nucleus of HCC cells with the increase of cell densities （30%, 50% and 90% confluence）; the result of Western blotting assay also showed that there was no obvious change in hnRNP K expression in the cytoplasm of HCC cells of different cell densities（30%, 50%, 70% and 90% confluence）. Conclusion: The expression of hnRNP K is elevated in HCC tissues and has an increasing tendency in the nucleus with the increase of cell density. Infection of HBV may be associated with the up-regulation of hnRNP K in HCC cells. |
| Key words: hepatocellular carcinoma heterogeneous nuclear ribonucleoprotein K hepatitis B virus |
