| 摘要: |
| 目的:观察CD40及基质金属蛋白酶-9(MMP9)在人颈动脉粥样硬化斑块中的表达，探讨CD40在斑块稳定性中的作用及可能机制。方法:将因颈动脉粥样硬化狭窄(>70%)行颈动脉内膜切除术的28例手术标本分为有临床卒中事件组(n=15)和无临床卒中事件组(n=13)，另设尸检无动脉粥样硬化的正常颈动脉(n=8)作为对照组，采用实时荧光定量聚合酶链反应(real-time PCR)检测各组斑块中CD40和MMP9 mRNA水平，Western印迹检测各组CD40和MMP9蛋白表达水平，并对CD40与MMP9基因的表达作相关性分析。结果:无临床卒中事件组和有临床卒中事件组的CD40、MMP9基因的表达较正常对照组明显增高（P＜0.01），有临床卒中事件组明显高于无临床卒中事件组（P＜0.01），CD40与MMP9 mRNA表达有正的直线相关关系（r=0.964，P＜0.01）。正常颈动脉中MMP9和CD40蛋白基本不表达；颈动脉粥样硬化斑块中MMP9和CD40蛋白表达较正常颈动脉明显增高；有卒中事件组颈动脉斑块MMP9和CD40蛋白表达高于无卒中事件组。结论:颈动脉粥样硬化斑块中CD40、MMP9表达增高，可能与斑块的稳定性密切相关。 |
| 关键词: CD40抗原 基质金属蛋白酶9 颈动脉疾病 动脉硬化 斑块稳定性 |
| DOI：10.3724/SP.J.1008.2008.01065 |
| 投稿时间：2008-02-26修订日期：2008-02-26 |
| 基金项目:上海市科学技术发展基金(03DZ19708);第二军医大学博士研究生创新性科学研究资助项目 |
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| Expression of CD40 and matrix metalloproteinase 9 in carotid atherosclerotic plaques and its significance |
| WANG Jing-hua1, DING Su-ju1*, DENG Ben-qiang1, WU Tao1, CHEN Huan2, ZHANG Sheng-sheng2, JIAO Bing-hua2 |
| (1. Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China*2. Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective:To investigate the expression of CD40 and MMP9 in carotid atherosclerotic plaques, so as to assess the role of CD40 in the stability of the plaque and the possible mechanism. Methods: The expression of CD40 and MMP9 mRNA and protein in carotid atherosclerotic plaques obtained from carotid eversion endarterectomy (CEE) of 28 patients with high-grade stenosis (>70%) (stroke group, n=15; non-stroke group, n=13) and 8 normal postmortem arteries (control group) were detected by real-time quantification polymerase chain reaction(PCR) and Western blotting. The correlation between expression of CD40 and MMP9 was analyzed. Results: The expression of CD40 mRNA and MMP9 mRNA in the non-stroke group and stroke group was significantly higher than that in control group (P＜0.01); and that of the stroke group was significantly higher than that of the non-stroke group (P＜0.01). There was a linear correlation between expression of CD40 and MMP9 mRNA (r=0.964，P＜0.01). Extremely rare expression of CD40 and MMP9 protein was found in the normal carotid artery; the protein expression was evidently higher in the carotid artery atherosclerosis than in the normal carotid artery, and the expression in carotid artery atherosclerosis in the stroke group was higher than that in the non-stroke group. Conclusion: The increased expression of CD40 and MMP9 in the carotid atherosclerositic plaques may be closely related to the stability of plaques. |
| Key words: CD40 antigen matrix metalloproteinase-9 carotid artery diseases artherosclerosis plaque stability |
