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| 前列腺癌组织中谷胱甘肽S-转移酶P1基因启动子区域甲基化序列分析 |
| 杜稳斌1,侯建国1*,武旗1,常文军2,翟羽佳2,林丽萍2,曹廷虎1,孙浚雯2,张宏伟2*,曹广文2 |
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| (1．第二军医大学长海医院泌尿外科,上海 200433;2．第二军医大学卫生勤务学系流行病学教研室,上海 200433) |
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| 摘要: |
| 目的：研究前列腺癌(prostate carcinoma,PCa)组织中谷胱甘肽S-转移酶P1 (glutathione S-transferase P1,GSTP1)基因启动子区域CpG岛甲基化状态,探索前列腺癌早期诊断的检测方法。方法：采用巢式甲基化特异性聚合酶链反应(nest methylation-specific PCR,NMSP)和基因克隆测序的方法检测31例前列腺癌组织、18例前列腺增生 (benign prostatic hyperplasia,BPH) 组织和3例正常前列腺(normal prostate,NP)组织中GSTP1基因启动子区域CpG岛的甲基化状态。结果：NMSP结果显示在PCa、BPH以及NP组中甲基化阳性率分别为83%、0%和0%；测序结果中PCa与BPH、NP组CG位点甲基化发生率分别为96%、34%和37%(P<0.01),BPH和NP组无统计学差异(P>0.05)。联合NMSP筛查前列腺癌明显优于单纯前列腺特异性抗原(prostate-specific antigen,PSA)检测。结论：在前列腺癌组织中GSTP1基因启动子区域CpG岛呈现超甲基化的状态,应用NMSP方法对GSTP1基因进行甲基化检测具有高灵敏度和高特异性的特点,这有望成为新的前列腺癌早期筛选和诊断的检测方法。 |
| 关键词: 前列腺癌 谷胱甘肽转移酶 启动区 甲基化 |
| DOI：10.3724/SP.J.1008.2008.00762 |
| 投稿时间：2008-02-27修订日期：2008-03-31 |
| 基金项目:国家自然科学基金(30671793). |
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| Promoter region methylation analysis of glutathione S-transferase p1 gene in prostate carcinoma |
| DU Wen-bin1,HOU Jian-guo1*,WU Qi1,CHANG Wen-jun2,ZHAI Yu-jia2,LIN Li-ping2,CAO Ting-hu1,SUN Jun-wen2,ZHANG Hong-wei2*,CAO Guang-wen2 |
| (1．Department of Urology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2．Department of Epidemiology,Faculty of Medical Services,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective：To investigate the promoter region CpG island methylation status of glutathione S-transferase p1(GSTP1) gene in prostate carcinoma (PCa) tissues and to search for new molecular markers for the early diagnosis of PCa.Methods: The promoter region CpG island methylation status of 31 PCa,18 benign prostatic hyperplasia tissues (BPH) and 3 normal prostate tissues (NP) were examined by nest methylation-specific PCR (NMSP) technique,cloning and sequencing.Results: NMSP showed that the methylation rates of PCa,BPH and NP tissues were 83%,0% and 0%,respectively.The CG sites methylation rates of PCa,BPH and NP tissues were 96%,34% and 37%,respectively as determined by cloning and sequencing (P<0.01),with no significant difference found between those of BPH and NP tissues(P>0.05).A combination of NMSP and prostate-specific antigen(PSA)was better than PSA alone in screen of PCa.Conclusion: CpG islands of promoter region of GSTP1 gene is hypermethylated in PCa tissues.NMSP is sensitive and specific in detection of GSTP1 methylation,which may serve as a new method for early screen of prostate carcinoma. |
| Key words: prostatic neoplasms glutathione transferase promoter regions methylation |
