| 摘要: |
| 目的:观察嗜酸乳杆菌LA14菌株耗尽培养上清液 (spent culture supernatant,SCS)对兔离体肠平滑肌收缩的影响，并初步探讨其作用机制。方法:制备兔离体回肠标本，分别记录肠平滑肌的正常收缩频率和幅度作为给药前对照，然后按累积剂量分别加入嗜酸乳杆菌SCS、活菌菌液、SCS+活菌菌液。每次给药0.3 ml，给药间隔6 min，在每次给药4 min后开始描记收缩曲线，记录时间2 min。观察嗜酸乳杆菌各治疗组对离体肠平滑肌收缩的作用。另取肠段按毛果芸香碱、阿托品或SCS、再毛果芸香碱的顺序给药，观察嗜酸乳杆菌SCS对M型胆碱受体的作用。结果:按累积剂量连续给予SCS或SCS+活菌菌液0.6～1.5 ml后，兔离体肠平滑肌收缩频率明显降低，与给药前比较差异有统计学意义（P<0.05或P<0.01），其余各点与给药前比较均无统计学差异。SCS、活菌菌液、SCS+活菌菌液在0.3～1.5 ml剂量范围内，仅SCS+活菌菌液在给药达1.5 ml时，兔离体肠平滑肌收缩幅度的降低与给药前比较有统计学差异（P<0.05），其余各点均无统计学差异。SCS或阿托品可明显对抗毛果芸香碱引起的兔离体肠平滑肌收缩幅度的增加（P<0.05或P<0.01），SCS还能使兔离体肠平滑肌收缩频率明显减少（P<0.01）。结论:嗜酸乳杆菌SCS可能通过阻断M型胆碱受体抑制兔离体肠平滑肌蠕动。 |
| 关键词: 嗜酸乳杆菌 耗尽培养上清液 肠平滑肌 M型胆碱受体 |
| DOI：10.3724/SP.J.1008.2009.0690 |
| 投稿时间：2008-09-02修订日期：2009-01-16 |
| 基金项目:大连大学本科生创新教育基金（061520B）. |
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| Spent culture supernatant of Lactobacillus acidophilus influences contraction of isolated intestinal smooth muscle of rabbits |
| LUO Xue-ya1*, LIU Bin2, ZHANG Cheng2, XIONG Mu-jun2, JIAO Xu-mei2 |
| (1.Department of Pharmacology, Medical College of Dalian University, Dalian 116622,China;2.Department of Clinical Medicine, Medical College of Dalian University, Dalian 116622) |
| Abstract: |
| Objective:To observe the influence of spent culture supernatant (SCS) of Lactobacillus acidophilus strain LA14 on the contraction of isolated intestinal smooth muscle and to discuss the related mechanism.Methods: The ileum samples of rabbits were prepared and the contraction frequency and amplitude of intestinal smooth muscle were observed as the normal control.Then the SCS, bacterium suspension, and SCS with bacterium suspension were added by an accumulative dose to the culture media (0.3 ml per times, at an interval of 6 min), respectively.Four minutes after each administration, the contractive curves were recorded for 2 min.The influences of various groups of Lactobacillus acidophilus on the contraction of isolated intestinal smooth muscle were observed.The effect of SCS on M cholinoceptor was observed by adding in order pilocarpine, atropine or SCS, and pilocarpine.Results: After continuous administration of SCS or SCS with bacterium suspension (0.6-1.5 ml), the contraction frequency of the intestinal smooth muscle was significantly lowered compared with before administration（P<0.05 or P<0.01），and there were no significant differences between before administration and other volume groups（P>0.05）.Within the range of 0.3-1.5 ml, the SCS, bacterium suspension, and SCS with bacterium suspension resulted in no significant difference in reducing the contraction amplitude, except for SCS with bacterium suspension at 1.5 ml（P<0.05）.SCS or atropine significantly inhibited pilocarpine-induced increase of contraction amplitude（P<0.05 or P< 0.01）.SCS also reduced the contraction frequency of the intestinal smooth muscle（P<0.01）.Conclusion: SCS of Lactobacillus acidophilus may inhibit the peristalsis of the intestinal smooth muscle of rabbits by blocking M cholinoceptor. |
| Key words: Lactobacillus acidophilus spent culture supernatant(SCS) intestinal smooth muscle M cholinoceptor |
