| 摘要: |
| 目的:研究血管内皮生长因子(VEGF)在血管外支架预防移植静脉再狭窄中的作用及不同管径材料外支架的作用效果。方法:通过动物实验获得外支架干预下的移植静脉及其对照标本,用免疫组化及形态学方法对标本中的VEGF、增殖细胞核抗原(PCNA)及滋养血管的表达进行检测、分析。结果:与对照组相比,外支架干预的移植静脉组中膜VEGF含量显著降低(P<0.05)，而外膜VEGF含量显著增高(P<0.05),中膜PCNA含量显著降低(P<0.05),外膜滋养血管密度显著增高(P<0.05)。结论:血管外支架可有效促进VEGF向外膜聚集,减少其在中膜、内膜聚集,进而促进滋养血管的生长,减少中膜增生,减轻再狭窄。 |
| 关键词: 血管内皮生长因子类 移植静脉 血管外支架 血管移植物闭塞 |
| DOI：10.3724/SP.J.1008.2009.0509 |
| 投稿时间：2008-09-05修订日期：2008-10-28 |
| 基金项目: |
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| Role of vascular endothelial growth factor in vein sheath prevention of arterial bypass graft stricture |
| YU Yong-chao1,ZHAO Xue-wei2*,XU Zhi-fei2,HE Jin3,ZHOU Jun2 |
| (1.Teaching and Research Division of Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Department of Cardiothoracic Surgery,Changzheng Hospital,Second Military Medical University,Shanghai 200003; 3.Department of Pathology,Changzheng Hospital,Second Military Medical University,Shanghai 200003) |
| Abstract: |
| Objective:To study the role of vascular endothelial growth factor(VEGF) in vein sheath prevention of arterial bypass graft stricture and the efficacies of vein sheath of different diameters. Methods: The vein grafts and their specimens were obtained via animal experiment of sheath intervention. Immunohistochemistry method was used to examine the expression of VEGF and proliferating cell nuclear antigen (PCNA) and the status of nutrient vessel in the specimens,so as to investigate the function of VEGF. Results: Compared with the control group,the vein sheath intervention group had significantly lower VEGF content in the media (P<0.05) and higher content in the adventitia (P<0.05); it also had lower PCNA content in the media (P<0.05) and increased blood vessel density in the adventitia (P<0.05). Conclusion: Vein sheath can effectively increase the distribution of VEGF in the adventitia and decrease its distribution in the media and intima,thus promoting the growth of nutrient blood vessels and decreasing media hyperplasia and re-stenosis. |
| Key words: vascular endothelial growth factors vein grafts vein sheath vascular graft occlusion |
