| 摘要: |
| 目的:探讨Let-7c在乳腺浸润性导管癌中的表达情况及与各种临床病理特征的关系。方法:采用茎环RT-PCR方法检测40例乳腺浸润性导管癌及其对应的正常乳腺组织中Let-7c的表达，分析Let-7c表达与临床分期、淋巴结转移、增殖指数等临床病理特征的关系。结果:用茎环RT-PCR方法检测Let-7c表达的敏感性和特异性良好; Let-7c在乳腺癌组织中的表达显著低于正常组织(P＜0.05)；Let-7c的表达与乳腺癌的增殖指数有关(P＜0.05)，与雌孕激素受体状态亦有关，与是否绝经、乳腺癌的TNM分期、淋巴结转移未见显著统计学意义(P＞0.05)。在高增殖指数、雌孕激素受体阴性的肿瘤组Let-7c表达明显降低。结论:Let-7c在乳腺浸润性导管癌中的表达异常，可能和乳腺癌的发生和发展有关，有望成为乳腺癌新的预后指标及治疗靶点。 |
| 关键词: Let-7c基因 微MicroRNA 乳腺肿瘤 乳腺导管癌 |
| DOI：10.3724/SP.J.1008.2009.0400 |
| 投稿时间：2008-09-07修订日期：2009-03-14 |
| 基金项目:温州市卫生局课题(2007010). |
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| Expression of Let-7c in invasive ductal carcinoma of breast cancer and its clinical significance |
| SHEN Shu-rong1, SHI Jun-yi1*, SHEN Xian2, HUANG Guan-li2, XUE Xiang-yang2 |
| (1.Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433,China;2.Department of General Surgery, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000) |
| Abstract: |
| Objective:To investigate the expression of Let-7c in patients with invasive ductal carcinoma of the breast cancer and its correlation with the clinicopathologic features of the patients. Methods: The expression of Let-7c was examined in 40 tissues of invasive ductal carcinoma of the breast cancer and their matched non-tumor adjacent tissue specimens by stem-loop real-time RT-PCR. The correlation of the expression with the clinicopathological features of breast cancer, such as the clinical staging, lymphatic metastasis, and proliferation index, was analyzed. Results: The stem-loop realtime RT-PCR was sensitive and specific in detecting Let-7c expression. Expression of Let-7c in breast cancer tissues was significantly lower than that in the adjacent normal tissues(P＜0.05). Down-regulation of Let-7c expression was associated with higher proliferation index (Ki-67＞10%) (P=0.030) and the status of Estrogen/Progesterone receptor (P=0.040/0.034)in breast cancer patients. There was no significant association of Let-7c expression with menopause condition, breast cancer TNM clinical stage, or lymphatic metastasis(P＞0.05). Conclusion: Our results suggest Let-7c may play an important role in the development of breast cancer and the level of Let-7c expression may be a potential parameter in forecasting the progression and prognosis of breast cancer patients. |
| Key words: Let-7c gene MicroRNAs breast neoplasms breast ductal carcinoma |
