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| HPLC法同时测定中药丹参中水溶性和脂溶性成分的含量 |
| 娄子洋1,张海2,李翔3,朱臻宇3,赵亮2,张国庆2,柴逸峰3* |
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| (1.第二军医大学药学院分析测试中心,上海 200433*2.第二军医大学东方肝胆外科医院药材科,上海 200438*3.第二军医大学药学院药物分析学教研室,上海,200433) |
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| 摘要: |
| 目的:建立同时测定丹参中2种水溶性成分和2种脂溶性成分的方法。方法:HPLC-DAD法,采用Agilent Zorbax TC C18柱(4.6 mm×250 mm,5 μm),以甲醇2%醋酸为流动相进行梯度洗脱:0～15 min,30% B～40% B; 15～20 min,40% B～60% B; 20～25 min,60% B～90% B; 25～40 min,90% B。检测波长为281 nm,柱温:35℃。结果:迷迭香酸、丹酚酸B、隐丹参酮、丹参酮ⅡA浓度分别在3.76~120.20 μg·ml-1 (r=0.999 9)、34.20~1 094.5 μg·ml-1 (r=0.999 9)、0.64~20.32 μg·ml-1 (r=0.999 9)、1.02~32.72 μg·ml-1 (r=0.999 6)范围内呈良好的线性关系。4种成分精密度试验RSD <1%。48 h内稳定性RSD<1%。加样回收率为99.72%～100.63%。结论:该含量测定方法简便,分离效果好,能同时测定丹参中迷迭香酸、丹酚酸B、隐丹参酮及丹参酮ⅡA四种有效成分的含量,结果准确可靠。 |
| 关键词: 丹参 多成分含量测定 迷迭香酸 丹酚酸B 隐丹参酮 丹参酮ⅡA 高压液相色谱法 |
| DOI：10.3724/SP.J.1008.2009.0190 |
| 投稿时间：2008-09-06修订日期：2008-12-11 |
| 基金项目:国家“十一五”科技支撑计划课题(2006BAI08B03-07). |
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| HPLC simultaneously determines multiple hydrophilic and lipophilic bioactive components in Radix et Rhizoma Salviae Miltiorrhizae |
| LOU Zi-yang1, ZHANG Hai2, LI Xiang3, ZHU Zhen-yu3, ZHAO Liang2 , ZHANG Guo-qing2, CHAI Yi-feng3* |
| (1.Center of Drug Analysis and Testing, School of Pharmacy, Second Military Medical University, Shanghai 200433, China*2.Department of Pharmacy, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438*3.Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective:To develop a new method for the simultaneous determination of two hydrophilic components and two lipophilic components of Radix et Rhizoma Salviae Miltiorrhizae.Methods: The HPLC-DAD method was employed using a column of Agilent Zorbax TC C18 (4.6 mm×250 mm, 5 μm) with a mobile phase of methanol -2% acetic acid.The gradient elution program was as follow:0-15 min, 30% B-40% B; 15-20 min, 40% B-60% B; 20-25 min, 60% B-90% B;25-40 min, 90% B.The detection wavelength was set at 281 nm and the temperature was 35℃.Results: The linearity was obtained over 3.76-120.20 μg·ml-1(r=0.999 9) for rosmarinic acid, 34.20-109 4.5 μg·ml-1(r=0.999 9) for salviamolic acid B, 0.64-20.32 μg·ml-1(r=0.999 9) for clyptotanshinon,and 1.02-32.72 μg·ml-1(r=0.999 6) for tanshinone ⅡA.The RSDs of precision and stability of the sample were both less than 1% in 48 hours.The average recovery was between 99.72%-100.63%.Conclusion: The present method is simple and has satisfactory efficacy; it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas. |
| Key words: Radix et Rhizoma Salviae Miltiorrhizae multicomponent assaying rosmarinic acid salviamolic acid B clyptotanshinon tanshinone ⅡA high pressure liquid chromatography |
