| 摘要: |
| 目的:探讨钠氢交换抑制剂(sodium-hydrogen exchange inhibitor,NHEI)能否加强含钾通道开放剂(potassium channel opener,PCO)的超极化停搏保存液对移植供体心脏的保护作用,明确该组合对移植供心的保护程度。方法:在晶体液灌注的Langendorff模型上平衡30 min后,兔心被分为4组:(1)Cel组:继续接受另一个30 min的平衡灌注,用Celsior液停搏并保存8 h然后再灌注1 h；(2) Pin组:心脏用pinacidil(一种PCO)超极化液停搏并保存,余同Cel组；(3)Cel+Car组:第二个30 min平衡期和再灌注时对心脏使用10 μmol/L的cariporide(一种NHEI),余与Cel组相同；(4)Pin+Car组:心脏接受cariporide的处理和PCO超极化停搏并保存,余同Cel组。结果:Pin+Car组供心收缩功能恢复值显著高于Pin组\[(87.4±11.9)% vs (69.0±7.2)%,P<0.05\],达到Cel组水平\[(93.4±5.2)%,P>0.05\]。Cel组与Cel+Car组间各项指标均没有显著差异。各组间冠脉流量无显著差别。结论:NHEI能显著提高PCO超极化停搏液对移植供心的保护效果,二者联合使用的保护效果与Celsior液相当。 |
| 关键词: 钠氢交换抑制剂 钾通道开放剂 心脏移植 心肌保存 |
| DOI：10.3724/SP.J.1008.2009.0663 |
| 投稿时间：2008-10-13修订日期：2009-03-01 |
| 基金项目:湖南省自然科学基金(05JJ30052). |
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| Protective effect of hyperpolarized preservation solution combining sodium-hydrogen exchange inhibitor and potassium channel opener on donor heart |
| PENG Hao1,LI Jin2,HU Ying-bin2,TANG Hao2,HU Ye-rong2,ZHOU Xin-min2* |
| (1.Department of Thoracic and Cardiovascular Surgery,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Cardiothoracic Surgery,The Second Xiangya Hospital,Central South University,Changsha 410011) |
| Abstract: |
| Objective:To study whether sodium-hydrogen exchange inhibitor(NHEI)can enhance the protective effect of hyperpolarized preservation solution containing potassium channel opener (PCO),and the effect was compared with that of Celsior solution.Methods: After equilibrated on a crystalloid-perfused Langendorff model for 30 min,rabbit hearts were divided into four groups:(1) Cel group:receiving another 30 min of equilibration,8 h cold storage with Celsior solution and 1 h reperfusion; (2) Pin group:arrested and stored with pinacidil (a type of PCO) hyperpolarizing cardioplegia; (3) Cel+Car group:receiving the same treatment as Cel group except that 10 μmol/L cariporide (a type of NHEI) was administered during the second equilibration period and reperfusion; and (4) Pin+Car group:receiving cariporide treatment and hyperpolarized arrest and storage.Results: Recovery rate of systolic function in the Pin+Car group was significantly higher than that in the Pin group (\[87.4±11.9\]% vs \[69.0±7.2\]%,P<0.05) and comparable to that in the Cel group (\[93.4±5.2\]%,P>0.05 ).No significant differences were found in any parameters between Cel group and Cel+Car group.There were no differences in coronary flow among all groups.Conclusion: NHEI can greatly enhance the protective effect of PCO hperpolarized preservation solution on the donor heart,and the combination of them has a comparable protective effect to Celsior solution. |
| Key words: PENG Hao1,LI Jin2,HU Ying-bin2,TANG Hao2,HU Ye-rong2,ZHOU Xin-min2* |
