| 摘要: |
| 目的:探讨参附注射液(Shenfu injection,SFI)对重症急性胰腺炎(SAP)大鼠肠屏障功能障碍与二次打击的防护作用。方法:雄性Wistar大鼠54只,随机分为3组:SAP组(SAP,n=24),采用逆行十二指肠胰胆管注射5％牛黄胆酸钠溶液制备SAP大鼠模型;SAP+SFI组(n=24),建模前2 h先给予SFI 10 ml/kg 体质量腹腔注射;假手术组(SO,n=6)。建模成功后3、6、12、24 h,分别取血液和小肠、胰腺、肺脏、肝脏标本。光镜下观察小肠组织病理改变,检测各时段血浆TNF-α、IL-6和二胺氧化酶(diamine oxidase,DAO)活性,以及相应时段胰腺、肺脏、肝脏中髓过氧化物酶(MPO)含量。结果:SAP组各时间点血液TNF-α、IL-6、DAO均较SO组显著升高(P<0.01),6～24 h胰腺、肺脏、肝脏MPO较SO组显著升高(P<0.01);SAP+SFI组各时间点血液DAO、TNF-α和IL-6水平显著低于SAP组(P<0.05或P<0.01),6～24 h胰腺、肺脏、肝脏MPO显著低于SAP组(P<0.05或P<0.01)；SAP+SFI组建模后24 h小肠病理组织改变较SAP组明显减轻。结论:SFI可防护SAP大鼠肠屏障功能障碍,并可减轻胰腺、肺脏、肝脏遭受二次打击的严重程度,其作用机制可能与减少中性粒细胞聚集、抑制促炎细胞因子TNF-α、IL-6相关。 |
| 关键词: 参附注射液 重症急性胰腺炎 肠屏障功能障碍 二次打击 细胞因子 |
| DOI：10.3724/SP.J.1008.2009.0913 |
| 投稿时间：2008-11-20修订日期：2009-07-10 |
| 基金项目:福建省自然科学基金(2006J0372). |
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| Protective effect of Shenfu injection against intestinal barrier dysfunction and second hit in rats with severe acute pancreatitis |
| ZOU Zhong-dong,ZHANG Zai-zhong,TANG Li-jun,TU Xiao-huang,WANG Lie,WANG Yu* |
| (Research Institute of General Surgery,Fuzhou General Hospital,PLA Nanjing Military Area Command,Fuzhou 350025,China) |
| Abstract: |
| Objective:To evaluate the protective effect of Shenfu injection (SFI) against intestinal barrier dysfunction and second hit in rats with severe acute pancreatitis (SAP).Methods:Fifty-four male Wistar rats were randomly divided into sham operation group (n=6),SAP group (n=24),and SAP+SFI group (10 ml/kg body wt,n=24).Sham operation group underwent laparotomy only.SAP model was established by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct of Wistar rats.SAP+SFI group was given SFI (10 ml/kg) intaperitoneally 2 h before SAP establishment.Rats were sacrificed at 3,6,12 and 24 hours after operation.The samples of pancreas,lung and liver were collected for measuring the levels of myeloperoxidase (MPO) at 3,6,12,and 24 h after injection of sodium taurocholate.The pathological changes of the terminal ilea were observed under light microscopy,and the blood levels of diamine oxidase (DAO),tumor necrosis factor alpha (TNF-α) and interleukin-6(IL-6) were also measured at different time points.Results:The blood levels of TNF-α,IL-6 and DAO in SAP group were significantly higher than those in the sham operation group at all time points (P<0.01).The levels of intrapulmonary,intrapancreatic and intrahepatic MPO in SAP group were significantly than those in the sham operation group at 6,12,and 24 hours (P<0.01).The contents of DAO,TNF-α and IL-6 were significantly decreased in SAP+SFI group compared with in SAP group at all time points(P<0.05 or P<0.01).The contents of intrapulmonary,intrapancreatic and intrahepatic MPO were significantly decreased in SAP+SFI group compared with SAP group at 6,12, and 24 h after operation(P<0.05 or P<0.01).Intestinal pathological damages were obviously milder in SAP+SFI group than that in SAP group at 24 h after operation.Conclusion:SFI can protect the small intestine mucosal barrier and other organs from second hit by reducing the polymorphonuclear leucocyte detaining and inhibiting TNF-α and IL-6. |
| Key words: Shenfu injection severe acute pancreatitis intestine barrier functional disturbance second attack cytokines |
